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B-hCXCR4 mice
Strain Name
C57BL/6-Cxcr4tm1(CXCR4)Bcgen/Bcgen 
Common Name  B-hCXCR4 mice
Background C57BL/6 Catalog number  110100
Related Genes 
CXC-R4, CXCR-4, CD184
NCBI Gene ID
12767

mRNA expression analysis

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Strain specific analysis of CXCR4 gene expression in WT and B-hCXCR4 mice by RT-PCR. Mouse Cxcr4 mRNA was detectable in splenocytes and thymocytes of wild-type (+/+). Human CXCR4 mRNA was detectable only in H/H, but not in +/+ mice. 

Protein expression analysis in T cells

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Strain specific CXCR4 expression analysis in homozygous B-hCXCR4 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCXCR4 (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific CXCR4 antibody. Mouse CXCR4 was detectable in WT mice. Human CXCR4 was exclusively detectable in homozygous B-hCXCR4, but the expression level was significantly lower than mouse CXCR4 in the C57BL/6 mice.


Protein expression analysis in CD4+ T cells

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Strain specific CXCR4 expression analysis in homozygous B-hCXCR4 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCXCR4 (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific CXCR4 antibody. Mouse CXCR4 was detectable in WT mice. Human CXCR4 was exclusively detectable in homozygous B-hCXCR4, but the expression level was significantly lower than mouse CXCR4 in the C57BL/6 mice. 

Protein expression analysis in B cells

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Strain specific CXCR4 expression analysis in homozygous B-hCXCR4 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCXCR4 (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific CXCR4 antibody. Mouse CXCR4 was detectable in WT mice. Human CXCR4 was exclusively detectable in homozygous B-hCXCR4, but the expression level was significantly lower than mouse CXCR4 in the C57BL/6 mice.