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B-hCSF1/hCSF1R mice
Strain Name
C57BL/6-Csf1tm1(CSF1)Begen Csf1rtm1(CSF1R)Begen/Bcgen
Common Name  B-hCSF1/hCSF1R mice
Background C57BL/6 Catalog number  121109
Related Genes 
MCSF; CSF-1
BANDDOS, C-FMS, CD115, CSF-1R, CSFR, FIM2, FMS, HDLS, M-CSF-R
NCBI Gene ID
12977,12978

Protein expression analysis

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Strain specific CSF1 expression analysis in homozygous B-hCSF1/hCSF1R mice by ELISA.Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCSF1/hCSF1R mice (H/H) stimulated with LPS in vivo, and analyzed by ELISA with species-specific anti-CSF1 ELISA kit. Mouse CSF1 was detectable in wild-type mice. Human CSF1 was exclusively detectable in homozygous B-hCSF1/hCSF1R but not in wild-type mice.


Protein expression analysis

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Strain specific CSF1R expression analysis in homozygous B-hCSF1/hCSF1R mice by flow cytometry.Blood were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCSF1/hCSF1R mice (H/H), and analyzed by flow cytometry with species-specific anti-CSF1R antibody. Mouse CSF1R was detectable in wild-type mice. Human CSF1R was exclusively detectable in homozygous B-hCSF1/hCSF1R but not in wild-type mice.


Combination therapy of anti-mouse PD-L1 antibody and anti-human CSF1R antibody


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Antitumor activity of anti-mouse PD-L1 antibody combined with anti-human CSF1R antibody in B-hCSF1/hCSF1R mice. (A) Anti-mouse PD-L1 antibody combined with anti-human CSF1R antibody inhibited hCSF1-MC38 tumor growth in B-hCSF1/hCSF1R mice. Murine colon cancer hCSF1-MC38 cells (5Χ105) were subcutaneously implanted into homozygous B-hCSF1/hCSF1R mice (female, n=5). Mice were grouped on the first day, at which time they were treated with anti-mouse PD-L1 antibody and anti-human CSF1R antibody with doses and schedules indicated in panel A. (B) Body weight changes during treatment. As shown in panel A, anti-human CSF1R antibody and combination of anti-mouse PD-L1 antibody and anti-human CSF1R antibody were efficacious in controlling tumor growth in B-hCSF1/hCSF1R mice, demonstrating that the B-hCSF1/hCSF1R mice provide a powerful model for in vivo evaluation of anti-human CSF1R antibodies and their combination with PD-L1 antibodies. Values are expressed as mean ± SEM.


In vivo efficacy of anti-human CSF1R antibodies 


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Antitumor activity of anti-human CSF1R antibodies in B-hCSF1/hCSF1R mice. (A) Anti-human CSF1R antibodies inhibited MC38 tumor growth in B-hCSF1/hCSF1R mice. Murine colon cancer MC38 cells were subcutaneously implanted into homozygous B-hCSF1/hCSF1R mice (female, 6-7-week-old, n=6). Mice were grouped when tumor volume reached approximately 100 mm3, at which time they were treated with two anti-human CSF1R antibodies with doses and schedules indicated in panel. (B) Body weight changes during treatment. (C) Mouse survival after grouping. As shown in panel A and C, anti-human CSF1R antibodies were efficacious in controlling tumor growth in B-hCSF1/hCSF1R mice, demonstrating that the B-hCSF1/hCSF1R mice provide a powerful preclinical model for in vivo evaluation of anti-human CSF1R antibodies. Values are expressed as mean ± SEM.


In vivo efficacy of anti-human CSF1R antibodies 



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