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B-hIL2/hIL2RA/hIL2RB mice
Strain Name
C57BL/6-Il2tm1(IL2)Bcgen Il2ratm1(IL2RA)Bcgen ll2rbtm2(IL2RB)Bcgen/Bcgen
       Common Name  B-hIL2/hIL2RA/hIL2RB mice
Background C57BL/6        Catalog number  131076
Related Genes 
IL2 also known as TCGF, lymphokine
IL2RA also known as CD25, IDDM10, IL2R, IMD41, TCGFR, p55
IL2RB also known as CD122.
NCBI Gene ID
16183,16184,16185

Protein expression analysis

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Strain specific IL2 expression analysis in homozygous B-hIL2/hIL2RA/ hIL2RB mice by ELISA. Serum was collected from wild-type mice (+/+) and homozygous B-hIL2/hIL2RA/ hIL2RB mice (H/H) stimulated with anti-mCD3 and anti-mCD28 in vivo, and analyzed by ELISA with species-specific IL2 ELISA kit. Mouse IL2 was detectable in wild-type mice. Human IL2 was exclusively detectable in homozygous B-hIL2/hIL2RA/ hIL2RB mice but not in wild-type mice.(ND: Not detectable)


IL2RA protein expression in T cells

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Strain specific IL2RA expression analysis in homozygous B-hIL2/hIL2RA/hIL2RB mice (H/H) by flow cytometry. Splenocytes were collected from wild-type mice (+/+) and homozygous B-hIL2/hIL2RA/hIL2RB mice (H/H) stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with anti-IL2RA antibody. Mouse IL2RA was detectable in T cells of wild-type mice. Human IL2RA was exclusively detectable in T cells of homozygous B-hIL2/hIL2RA/hIL2RB (H/H) but not in wild-type mice by the species-specific anti-IL2RA antibody.

IL2RB protein expression in T cells

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Strain specific IL2RB expression analysis in homozygous B-hIL2/hIL2RA/hIL2RB mice (H/H) by flow cytometry. Splenocytes were collected from wild-type mice (+/+) and homozygous B-hIL2/hIL2RA/hIL2RB mice (H/H) stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with anti-IL2RB antibody. Mouse IL2RB was detectable in T cells of wild-type mice. Human IL2RB was exclusively detectable in T cells of homozygous B-hIL2/hIL2RA/hIL2RB (H/H) but not in wild-type mice by the species-specific anti-IL2RB antibody.

Analysis of leukocytes subpopulation in B-hIL2/hIL2RA/hIL2RB mice

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Analysis of leukocyte subpopulations by FACS. Splenocytes, lymph node and blood were isolated from C57BL/6 and B-hIL2/hIL2RA/hIL2RB mice (female, n=3, 8-week-old). Flow cytometry analysis were performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, granulocytes, monocytes in homozygous B-hIL2/hIL2RA/hIL2RB mice were similar to those in the C57BL/6 mice, demonstrating that hIL2/hIL2RA/hIL2RB humanized does not change the overall development, differentiation or distribution of these cell types. Data of lymph node and blood cells were not shown. Values are expressed as mean ± SEM.

Analysis of T cell subpopulations in B-hIL2/hIL2RA/hIL2RB mice

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Analysis of T cell subpopulations by FACS. Splenocytes, lymph node and blood were isolated from C57BL/6 and B-hIL2/hIL2RA/hIL2RB mice (female, n=3, 8-week-old). Flow cytometry analysis were performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for TCRβ+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells, CD4+ T cells and Tregs in homozygous B-hIL2/hIL2RA/hIL2RB mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hIL2/hIL2RA/hIL2RB in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell subtypes. Data of lymph node and blood cells were not shown. Values are expressed as mean ± SEM.