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B-hIL3 mice
Strain Name
C57BL/6-Il3tm1(IL3)Bcgen/Bcgen  
Common Name  B-hIL3 mice
Background C57BL/6 Catalog number 110080
Aliases 
IL-3, MCGF, MULTI-CSF
NCBI Gene ID
16187

mRNA expression analysis

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Strain specific analysis of IL3 gene expression in wild-type C57BL/6 mice and homozygous B-hIL3 mice by RT-PCR. Mouse splenocytes were collected and stimulated with ConA for 48 hrs in vitro. Mouse Il3 mRNA was detectable in wild-type C57BL/6 mice (+/+). Human IL3 mRNA was detectable only in homozygous B-hIL3 mice (H/H) but not in wild-type mice. 

Protein expression analysis

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Strain specific IL3 expression analysis in homozygous B-hIL3 mice by ELISA. Splenocytes culture supernatant was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hIL3 mice (H/H) stimulated with PMA/ionomycin in vitro, and analyzed by ELISA with species-specific IL3 ELISA kit. Mouse IL3 was detectable in wild-type mice. Human IL3 was exclusively detectable in homozygous B-hIL3 mice but not wild-type mice.