B-hMASP2 mice
| Strain Name | C57BL/6-Masp2tm1(MASP2)Bcgen/Bcgen | Common Name | B-hMASP2 mice |
| Background | C57BL/6 | Catalog number | 110872 |
|
Related Genes |
sMAP; MAP19; MASP-2; MASP1P1 | ||
Protein expression analysis
Strain specific MASP2 expression analysis in heterozygous B-hMASP2 mice by ELISA. Serum were collected from C57BL/6 and heterozygous B-hMASP2 mice, and analyzed by ELISA with species-specific MASP2 ELISA kit. Mouse MASP2 was detectable in C57BL/6 and B-hMASP2 mice. Human MASP2 was exclusively detectable in B-hMASP2 mice but not in C57BL/6 mice.
Strain specific analysis of MASP2 gene expression in wild-type C57BL/6 mice and hMASP2 mice by RT-PCR. Human MASP2 mRNA was detectable in homozygous B-hMASP2 mice (H/H), but not in wild-type C57BL/6 mice (+/+). Mouse MASP2 mRNA was both detectable in wild-type C57BL/6 and homozygous B-hMASP2 mice.
Strain specific MASP2 expression analysis in homozygous B-hMASP2 mice by ELISA. Serum were collected from C57BL/6 and homozygous B-hMASP2 mice, and analyzed by ELISA with species-specific MASP2 ELISA kit. Mouse MASP2 was detectable in C57BL/6 and B-hMASP2 mice. Human MASP2 was not detectable in B-hMASP2 mice.
Analysis of spleen leukocyte subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and B-hMASP2 mice (n=3, 6-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hMASP2 mice were similar to those in the C57BL/6 mice, demonstrating that MASP2 humanized does not change the overall development, differentiation or distribution of these cell types in spleen.
Analysis of spleen T cell subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and B-hMASP2 mice (n=3, 6-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for TCRβ+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells , CD4+ T cells and Tregs in homozygous B-hMASP2 mice were similar to those in the C57BL/6 mice, demonstrating that MASP2 humanized does not change the overall development, differentiation or distribution of these cell types in spleen.
Analysis of lymph node leukocyte subpopulations by FACS. Lymph nodes were isolated from female C57BL/6 and B-hMASP2 mice (n=3, 6-week-old). Flow cytometry analysis of the lymph node was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells in homozygous B-hMASP2 mice were similar to those in the C57BL/6 mice, demonstrating that MASP2 humanized does not change the overall development, differentiation or distribution of these cell types in lymph node.
Analysis of lymph node T cell subpopulations by FACS. Lymph nodes were isolated from female C57BL/6 and B-hMASP2 mice (n=3, 6-week-old). Flow cytometry analysis of the lymph node was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for TCRβ+T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells , CD4+ T cells and Tregs in homozygous B-hMASP2 mice were similar to those in the C57BL/6 mice, demonstrating that MASP2 humanized does not change the overall development, differentiation or distribution of these cell types in lymph node.
Analysis of blood leukocyte subpopulations by FACS. Blood cells were isolated from female C57BL/6 and B-hMASP2 mice (n=3, 6-week-old). Flow cytometry analysis of the blood was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hMASP2 mice were similar to those in the C57BL/6 mice, demonstrating that MASP2 humanized does not change the overall development, differentiation or distribution of these cell types in blood.
Analysis of blood T cell subpopulations by FACS. blood cells were isolated from female C57BL/6 and B-hMASP2 mice (n=3, 6-week-old). Flow cytometry analysis of the blood was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for TCRβ+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells , CD4+ T cells and Tregs in homozygous B-hMASP2 mice were similar to those in the C57BL/6 mice, demonstrating that MASP2 humanized does not change the overall development, differentiation or distribution of these cell types in blood.
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