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B-hGPC3 mice
Strain Name
C57BL/6N-Gpc3tm1(GPC3)Bcgen/Bcgen 
Common Name  B-hGPC3 mice
Background C57BL/6N Catalog number 110873
Related Genes 
GPC3, DGSX, GTR2-2, MXR7, OCI-5, SDYS, SGB, SGBS, SGBS1, glypican 3
NCBI Gene ID
14734

mRNA expression analysis

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Strain specific analysis of GPC3 gene expression in wild type (WT) mice and B-hGPC3 mice by RT-PCR. Mouse Gpc3 mRNA was detectable only in kidney of WT mice (+/+). Human GPC3 mRNA was detectable only in homozygous B-hGPC3 mice (H/H) but not in WT mice (+/+). 


Protein expression analysis

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Strain specific GPC3 expression analysis in homozygous B-hGPC3 mice by western blot. Kidney, heart and brain were collected from wild type (WT) mice (+/+) and homozygous B-hGPC3 mice (H/H), and analyzed by western blot with anti-GPC3 antibody. GPC3 was detectable in WT mice (+/+) and homozygous B-hGPC3 mice (H/H) due to the cross-reactivity of antibodies.