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B-hMERTK mice
Strain Name
C57BL/6-Mertktm2(MERTK)Bcgen/Bcgen  
Common Name  B-hMERTK mice
Background C57BL/6N Catalog number  111278
Related Genes 
Mertk (Me; Ny; Eyk; Mer; Nyk; nmf1; nmf12)
NCBI Gene ID
17289

mRNA expression analysis

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Analysis of MERTK gene expression in wild-type mice and B-hMERTK mice by q-PCR. The mRNA expression of MERTK in B-hMERTK (H/H) was similar to those in the wild-type mice (+/+), demonstrating that introduction of hMERTK in place of its mouse counterpart does not change the expression level of MERTK mRNA.  


Protein expression analysis



Strain specific MERTK expression analysis in homozygous B-hMERTK mice by flow cytometry. Peritoneal washes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hMERTK mice (H/H). Flow cytometry analysis of the macrophage was analyzed with species-specific anti-MERTK antibody. Mouse MERTK was detectable in wild-type mice. Human MERTK was only detectable in homozygote B-hMERTK mice but not in wild-type mice.


Analysis of leukocytes cell subpopulation in spleen

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Analysis of spleen leukocyte subpopulations by FACS. Splenocytes were isolated from male C57BL/6 and B-hMERTK mice(n=3, 7-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hMERTK mice were similar to those in the C57BL/6 mice, demonstrating that B-hMERTK humanized does not change the overall development, differentiation or distribution of these cell types in spleen. Values are expressed as mean ± SEM.

Analysis of T cell subpopulation in spleen


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Analysis of spleen T cell subpopulations by FACS. Splenocytes were isolated from male C57BL/6 and B-hMERTK mice(n=3, 7-week-old).  Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells, CD4+ T cells, and Tregs in homozygous B-hMERTK mice were similar to those in the C57BL/6 mice, demonstrating that introduction of B-hMERTK in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell subtypes in spleen. Values are expressed as mean ± SEM.

Analysis of leukocytes cell subpopulation in lymph node


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Analysis of lymph node leukocyte subpopulations by FACS.Leukocytes were isolated from male C57BL/6 and B-hMERTK  mice(n=3, 7-week-old) Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis Percent of T cells, B cells, NK cells, in homozygous B-hMERTK mice were similar to those in the C57BL/6 mice, demonstrating that introduction of B-hMERTK in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in lymph node. Values are expressed as mean ± SEM.




Analysis of T cell subpopulation  lymph node

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Analysis of lymph node leukocyte subpopulations by FACS. Leukocytes were isolated from male C57BL/6 and B-hMERTK mice(n=3, 7- week-old) Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. Representative FACS plots. Single live CD45+ cells were gated for TCRb+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells, CD4+ T cells in homozygous B-hMERTK mice were similar to those in the C57BL/6 mice,  demonstrating that introduction of B-hMERTK in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in lymph node. Values are expressed as mean ± SEM.


Analysis of leukocytes cell subpopulation in blood

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Analysis of blood leukocyte subpopulations by FACS. Leukocytes were isolated from male C57BL/6 and B-hMERTK mice(n=3, 7-week-old) Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hMERTK mice were similar to those in the C57BL/6 mice, demonstrating that introduction of B-hMERTKin place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in blood. Values are expressed as mean ± SEM.

Analysis of T cell subpopulation in blood

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Analysis of blood leukocyte subpopulations by FACS. Leukocytes were isolated from male C57BL/6 and B-hMERTKmice(n=3, 7-week-old) Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. Representative FACS plots. Single live CD45+ cells were gated for TCRb+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD4+, CD8+, Tregs in homozygous B-hMERTK mice were similar to those in the C57BL/6 mice,  demonstrating that introduction of B-hMERTKin place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in blood. Values are expressed as mean ± SEM.