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B-hPD-L1/hCD27 mice
Strain Name
C57BL/6-Cd274tm1(CD274)Bcgen Cd27tm1(CD27)Bcgen/Bcgen
Common Name  B-hPD-L1/hCD27 mice
Background C57BL/6 Catalog number  120537
Related Genes 
CD274 (CD274 molecule)
CD27 
(CD27 molecule)
NCBI Gene ID
60533,21940


Protein Expression Analysis


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Strain specific PD-L1 expression analysis in homozygous B-hPD-L1/hCD27 mice by flow cytometry. 

Splenocytes were collected from WT and homozygous B-hPD-L1/hCD27 mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-PD-L1 antibody. Mouse PD-L1 was exclusively detectable in WT mice. Human PD-L1 were exclusively detectable in B-hPD-L1/hCD27 but not WT mice.


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Strain specific CD27 expression analysis in homozygous B-hPD-L1/hCD27 mice by flow cytometry.

Splenocytes were collected from WT and homozygous B-hPD-L1/hCD27 mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-CD27 antibody. Mouse CD27 was detectable in WT and B-hPD-L1/hCD27 mice. Human CD27 were exclusively detectable in B-hPD-L1/hCD27 but not WT mice. This might result from the cross-recognition of hCD27 by anti-mCD27 antibodies.