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B-hPVRIG mice
Strain Name
C57BL/6-Pvrigtm1(PVRIG)Bcgen/Bcgen
Common Name  B-hPVRIG mice
Background C57BL/6 Catalog number 110091
Related Genes 
CD112R; C7orf15
NCBI Gene ID
102640920

mRNA expression analysis


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Strain specific analysis of PVRIG gene expression in WT and B-hPVRIG mice by RT-PCR. Mouse Pvrig mRNA was detectable in splenocytes of wild-type (+/+). Human PVRIG mRNA was detectable only in B-hPVRIG mice (H/H) but not in wild type mice.

Protein expression analysis 


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PVRIG expression analysis in homozygous B-hPVRIG mice by western blot. Heart collected from wild type and homozygous B-hPVRIG mice (H/H) and analyzed by western blot with anti-PVRIG antibody. Mouse PVRIG was detectable in wild-type mice. Human PVRIG was detectable in homozygous B-hPVRIG mice. Anti-PVRIG antibody is crossly reactive with PVRIG in human and mice.


Analysis of spleen leukocytes cell subpopulations in B-hPVRIG mice



Analysis of spleen leukocyte subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and B-hPVRIG mice (n=3, 8-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hPVRIG mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hPVRIG in place of its mouse counterpart dose not change the overall development, differentiation or distribution of these cell types in spleen. Values are expressed as mean ± SEM.


Analysis of spleen T cell subpopulations in B-hPVRIG mice



Analysis of spleen T cell subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and B-hPVRIG mice (n=3, 8-week-old).  Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD4+ T cells, CD8+ T cells and Treg cells in homozygous B-hPVRIG mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hPVRIG in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell subtypes in spleen. Values are expressed as mean ± SEM.


Analysis of blood leukocytes cell subpopulations in B-hPVRIG mice



Analysis of blood leukocyte subpopulations by FACS. Blood cells were isolated from female C57BL/6 and B-hPVRIG mice (n=3, 8-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hPVRIG mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hPVRIG in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in spleen. Values are expressed as mean ± SEM.


Analysis of blood T cell subpopulations in B-hPVRIG mice



Analysis of blood T cell subpopulations by FACS. Blood cells were isolated from female C57BL/6 and B-hPVRIG mice (n=3, 8-week-old).  Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD4+ T cells, CD8+ T cells and Treg cells in homozygous B-hPVRIG mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hPVRIG in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell subtypes in spleen. Values are expressed as mean ± SEM.


Analysis of Lymph node leukocytes cell subpopulations in B-hPVRIG mice



Analysis of lymph node leukocyte subpopulations by FACS. lymph node were isolated from female C57BL/6 and B-hPVRIG mice (n=3, 8- week-old) Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of T cells, B cells and NK cells in homozygous B-hPVRIG mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hPVRIG in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in lymph node. Values are expressed as mean ± SEM.


Analysis of Lymph node T cell subpopulations in B-hPVRIG mice


Analysis of lymph node T cell subpopulations by FACS. lymph node  were isolated from female C57BL/6 and B-hPVRIG mice (n=3, 8-week-old).  Flow cytometry analysis of the lymph node  was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD4+ T cells, CD8+ T cells and Treg cells in homozygous B-hPVRIG mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hPVRIG in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell subtypes in spleen. Values are expressed as mean ± SEM.


In vivo efficacy of anti-human PVRIG antibodies

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Antitumor activity of anti-human PVRIG antibodies in B-hPVRIG mice. (A) COM-701 (in house) and SRF-813 (in house) inhibited MC38 tumor growth in B-hPVRIG mice. Murine colon cancer MC38 cells were subcutaneously implanted into homozygous B-hPVRIG mice (female, 6-week-old, n=5). Mice were grouped when tumor volume reached approximately 100 mm3, at which time they were treated with COM-701 (in house) and SRF-813 (in house) with doses and schedules in panel A. (B) Body weight changes during treatment. As shown in panel A, anti-human PVRIG antibodies were efficacious in controlling tumor growth in B-hPVRIG mice, demonstrating that the B-hPVRIG mice provide a powerful preclinical model for in vivo evaluation of anti-human PVRIG antibodies. Values are expressed as mean ± SEM.


In vivo efficacy of anti-human PVRIG antibodies 


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