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B-hTNFR2/hTNFR1 mice

Strain Name
C57BL/6-Tnfrsf1btm1(TNFRSF1B)Bcgen Tnfrsf1atm1(TNFRSF1A)Bcgen/Bcgen
Common Name  B-hTNFR2/hTNFR1 mice
Background C57BL/6 Catalog number  120802
Related Genes 
TNFRSF1B (Tumor necrosis factor receptor superfamily, member 1b);
TNFRSF1A
(Tumor necrosis factor receptor superfamily, member 1a)
NCBI Gene ID
21938,21937

mRNA expression analysis


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Strain specific analysis of TNFR1 and TNFR2 gene expression in WT and B-hTNFR2/TNFR1 mice by RT-PCR. Mouse Tnfr1 and Tnfr2 mRNA were detectable only in splenocytes of wild-type (+/+) mice. Human TNFR1 and TNFR2 mRNA were detectable only in H/H, but not in +/+ mice.


Protein expression analysis

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Strain specific TNFR2 expression analysis in homozygous B-hTNFR2/hTNFR1 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hTNFR2/hTNFR1 (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-TNFR2 antibody. Mouse TNFR2 was detectable in WT mice. Human TNFR2 was exclusively detectable in homozygous B-hTNFR2/hTNFR1 but not WT mice.


Protein expression analysis

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Strain specific TNFR1 expression analysis in homozygous B-hTNFR2/hTNFR1 mice by flow cytometry. Splenocytes and blood were collected from WT and homozygous B-hTNFR2/hTNFR1 (H/H) mice, and analyzed by flow cytometry with species-specific anti-TNFR1 antibody. Mouse TNFR1 was detectable in the WT mice. Human TNFR1 was exclusively detectable in homozygous B-hTNFR2/hTNFR1 but not WT mice.