| Strain Name |
|
Stock No. | 110779 |
| Common name |
B-Cx3cr1-iCreERT2-EGFP mice |
Gene symbol and name | Cx3cr1,chemokine (C-X3-C motif) receptor 1 |
| Strain of origin |
C57BL/6N |
Chromosome | 9 |
| Coat color | Black | Dietary information | Growth and reproduction diet for experimental mice |
|
NCBI Gene ID |
13051 | ||
Application
- Function research of genes
- Function research of microglial cells and other Cx3cr1-expressing immune cells
Expression
Expressed gene
iCre/ERT2, improved cre recombinase and estrogen receptor 1 (human) fusion gene
Site of expression
monocytes, macrophage and brain microglia
Gene editing strategy
A targeting vector was designed to replace 390bp of exon 2 of the Cx3cr1 gene with a iCre/ERT2 (improved cre recombinase fulsed to estrogen receptor 1) coding sequence, followed by a porcine teschovirus-1 2A (P2A) and a enhanced green fluorescent protein (EGFP). The targeting vector were injected into C57BL/6N mouse zygotes. After injection, surviving 2-cell-stage zygotes were transplanted to the KM albino pseudopregnant females. The reslulting Cx3cr1-iCreERT2-EGFP founder mice were bred to C57BL/6N mice. These mice were maintained on a C57BL/6N background.
Phenotype Analysis
The
expression of EGFP and tdTomato in mouse spleen, bone marrow, and brain
mononuclear macrophages was detected by flow cytometry, and Cx3cr1 (Mut /+) was
found after induction of Tamoxifen.The majority of macrophages in the brain of
tdTomato (Mut/+) mice were double positive cells of EGFP and tdTomato, while the
mononuclear macrophages in spleen and bone marrow were single positive cells of
EGFP. The results were consistent with the results in the study, indicating
that B-Cx3cr1-iCreERT2-EGFP mice were designed and expressed successfully.
1. GoldmannTobias,WieghoferPeter,Müller Philippe F et al. A new type of microglia gene targeting shows TAK1 to be pivotal in CNS autoimmune inflammation.[J] .Nat. Neurosci., 2013, 16: 1618-26. [PMID: 24077561]
2. YonaSimon,Kim Ki-Wook,Wolf Yochai et al. Fate mapping reveals origins and dynamics of monocytes and tissue macrophages under homeostasis.[J] .Immunity, 2013, 38: 79-91. [PMID: 23273845]
3. Rogers J T ,Morganti J M , Bachstetter A D , et al. CX3CR1 Deficiency Leads to Impairment of Hippocampal Cognitive Function and Synaptic Plasticity[J]. Journal of Neuroscience, 2011, 31(45):16241-16250. [PMID: 22072675]
4. Ushijima T , Fujimoto N , Matsuyama S , et al. The actin-organizing formin protein Fhod3 is required for postnatal development and functional maintenance of the adult heart in mice[J]. Journal of Biological Chemistry, 2017:jbc.M117.813931. [PMID: 29158260]
5. Madisen L ,Zwingman T A , Sunkin S M , et al. A robust and high-throughput Cre reporting and characterization system for the whole mouse brain[J]. NATURE NEUROSCIENCE, 2009, 13(1):133-140. [PMID: 20023653]
