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B-hCD47 CT26.WT
Common name
B-hCD47 CT26.WT  Catalog number     310702
Aliases IAP, MER6, OA3 Disease  Colon carcinoma
Organism
Mouse
Strain  BALB/c
Tissue types Colon Tissue  Colon

Description 

The mouse Cd47 gene was replaced by human CD47 gene in B-hCD47 CT26.WT  cells. Human CD47 is highly expressed on the surface of B-hCD47 CT26.WT  cells.


Application


B-hCD47 CT26.WT  cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hCD47 CT26.WT  cells. The IgV domain on exon 2 of mouse Cd47 gene was replaced by the IgV domain of human CD47 on exon 2 in B-hCD47 CT26.WT . 

Protein expression analysis 

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CD47 expression analysis in B-hCD47 CT26.WT  cells by flow cytometry. Single cell suspensions from wild-type CT26.WT and B-hCD47 CT26.WT  cultures were stained with species-specific anti-CD47 antibody. Human CD47 was detected on the surface of B-hCD47 CT26.WT  cells but not wild-type CT26.WT cells. The 1-F12 clone of B-hCD47 CT26.WT  cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hCD47 CT26.WT  cells. B-hCD47 CT26.WT  cells (1x105) and wild-type CT26.WT cells (1x105) were subcutaneously implanted into BALB/c mice (female, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCD47 CT26.WT  cells were able to establish tumors in vivo and can be used for efficacy studies.