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B-hSIGLEC15 MC38
Common name
B-hSIGLEC15 MC38 Catalog number    311049
Aliases CD33L3, HsT1361, SIGLEC-15 Disease  Colon carcinoma
Organism
Mouse
Strain  C57BL/6
Tissue types Colon Tissue  Colon

Description 


The mouse Siglec15 gene was replaced by human SIGLEC15 coding sequence in B-hSIGLEC15 MC38 cells. Human SIGLEC15 is highly expressed on the surface of B-hSIGLEC15 MC38 cells.

Application

B-hSIGLEC15 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hSIGLEC15 MC38.The partial exon 3~3’UTR of mouse Siglec15 gene were knocked out and human SIGLEC15 CDS was insert into mouse Siglec15 exon 3 in B-hSIGLEC15 MC38. 

Protein expression analysis 

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The SIGLEC15 expression analysis in B-hSIGLEC15 MC38 cells by flow cytometry. Single cells were collected from wild-type MC38 and B-hSIGLEC15 MC38 cells, and analyzed by flow cytometry with species-specific anti-SIGLEC15 antibody (in house). Human SIGLEC15 was exclusively detectable in B-hSIGLEC15 MC38 cells but not wild-type MC38 cells. The 3-D10 clone of B-hSIGLEC15 MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hSIGLEC15 MC38 cells. B-hSIGLEC15 MC38 cells (1x106) and wild-type MC38 cells (5x105 ) were subcutaneously implanted into the B-hSIGLEC15 mice (female, 8-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hSIGLEC15 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.