바이오사이토젠의 고도화 면역 결핍 마우스인 B-NDG 마우스는 인간 PBMC와 hHSC (CD34+ hematopoietic stem cells)를 이식하기에 좋은 도구입니다. 이 인간화 면역 세포가 이식된 모델들은 인간 면역 세포에서 면역항암제의 효과를 검증하기 위한 강력한 도구이며, CDX 와 PDX 모델에서의 효능 평가를 위해서도 유용합니다. 아울러 이 모든 CDX 모델에 대하여 PD(pharmacodynamic 서비스 또한 제공하고 있습니다.
Cell lines Tumor type Cell lines Tumor type Human solid tumor cell lines NCI-H1975 Lung carcinoma, non-small cell ZR-75-1 Breast ductal carcinoma HCC827 Lung adenocarcinoma MDA-MB-231 Breast adenocarcinoma NCI-H520 Lung carcinoma DU4475 Breast carcinoma, triple negative A549 Lung carcinoma MCF7 Mammary gland, breast adenocarcinoma NCI-H1781 Lung carcinoma bronchoalveolar 5637 Bladder grade II carcinoma HCT-8 Colorectal Ileocecal adenocarcinoma A431 Epidermoid carcinoma HCT-116 Colon carcinoma LN229 Glioblastoma Colo205 Colorectal adenocarcinoma Miapaca-2 Pancreatic carcinoma Human blood tumor cell lines Daudi Lymphoma, NAMALWA Burkitt’s lymphoma KG-1 Leukemia, acute myelogenous (AML) Kasumi-1 Leukemia, acute myeloblastic (AML) Raji Lymphoma, Burkitt’s SU-DHL-1 Large cell lymphoma, Diffuse histiocytic lymphoma K562 Leukemia (CML) MV4-11 Peripheral blood biphenotypic B myelomonocytic leukemia
루시퍼라아제가 추가된 세포주로 만들어진 CDX 모델
No. Cell name Cancer type B-HCL-013 B-luciferase-GFP Raji Cell Line Lymphoma, Burkitt’s B-MCL-001 B-luciferase-GFP HT-29 Cell Line Colorectal adenocarcinoma B-MCL-002 B-luciferase-GFP H-1975 Cell Line Lung carcinoma, non-small cell B-HCL-014 B-luciferase K562 Cell Line Leukemia (CML) B-HCL-015 B-luciferase MIA PaCa-2 Cell Line Pancreatic carcinoma B-HCL-010 B-luciferase U-87MG cell line Glioblastoma
CDX 마우스 모델에서의 TGK (Tumor Growth Kinetics)
Case 1: HT-29-GFP Luciferase 암세포를 주사한 후 6, 16 , 28일 후의 NOD-scid 마우스의 In vivo 이미지.
A) The mouse on the left was injected with 5 x 106 cells. The mouse on the right was injected with 5 x 105 (B) Signal intensity and tumor size graph.
Case 2: 4T1-Luciferase 암세포를 주사한 후 7, 14 , 21일 후의 NOD-scid 마우스의 In vivo 이미지.
(A) The mouse on the left was injected with 5 x 106 cells; the mice on right was injected with 5 x 105 cells. (B) Signal intensity and tumor size graph.
Case 3: 인간 B 세포 림프종 CDX 모델
Raji cells (5×10⁶) were injected into B-NDG, NOD-scid and BALB/C nude mice. (A) Kaplan-Merier survival curves. (B) Relative body weight change. (C) Percentage of human cells in mouse peripheral blood quantified by q-PCR. (D) Liver tumor nodules. (E) Immunohistochemical staining of livers and spleens.
CDX 약효 평가 결과 예시
Raji-Fluc cells (5 x 10⁵) were injected into B-NDG mice. Antibody X was administered on specified days. (A) Representative in vivo imaging recorded at different time points to monitor tumor progression. (B) Tumor load (as indicated by luciferase activity) kinetic curves post antibody treatment. Early dosing on day 3 in addition to day 10 resulted in robust tumor growth inhibition. In contrast, a single dose at day 10 had little effect.
Humanized B-NDG mice engrafted with human CD34+ HSC were intravenously injected with 5 x 10⁵ Raji-Fluc cells. Five days after Raji-Fluc cells implantation, mice were treated with anti-human PD-1 antibody. (A) Images of tumor load as indicated by luciferase activity. Tumor growth was dramatically inhibited by the human PD-1 antibody at day 7. RC=Raji-Fluc + Control. PDH=Antibody X + Humanized Mice. Naive B-NDG=Untreated Group.