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Mouse Immunodeficiency System CDX Model


Mouse Immunodeficiency System CDX Model


Xenotransplantation of human-derived tumor cell lines (CDX) refers to the tumor model established by inoculating human tumor cells into immunodeficient mice (such as severely immunodeficient B-NDG mice) after in vitro subculture. The commonly used inoculation sites are subcutaneous, orthotopic or intravenous. This tumor model has the advantages of good modeling stability and high success rate, and is widely used in tumor cell proliferation research and in the screening of anti-tumor drugs in vivo.

Using our self-developed immunodeficient B-NDG mice, Biocytogen establishes a variety of solid tumor models for anti-tumor drug screening, including lung cancer, rectal adenocarcinoma, colon cancer, breast cancer, pancreatic cancer, bladder cancer, liver cancer and a variety of hematological tumor models such as acute lymphoid leukemia and acute myeloid leukemia. Using these models, we  provide high-quality pharmacodynamic (PD) services.

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Xenograft model [1]



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Xenograft Model [2]


Case 1: Melanoma Model


Human melanoma A2058 cells were inoculated subcutaneously in B-NDG mice to successfully establish a melanoma CDX model.


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A. Tumor growth curve, B. Body weight change of tumor-bearing mice


Case 2: Human B-cell lymphoma model


The B cell lymphoma model was successfully established by injecting Raji cells into B-NDG, NOD-scid and BALB/C nude mice via the tail vein.


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(A) Kaplan-Merier survival curves, (B) relative body weight change, (C) q-PCR quantification of peripheral blood human cell ratios in mice, (D) liver tumors, (E) immunohistochemical staining of liver and spleen


Case 3: Hepatoma Model


Human hepatoma HuH-7 cells were inoculated subcutaneously in B-NDG mice to successfully establish a hepatoma tumor model.


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A. Tumor growth curve, B. Body weight change of tumor-bearing mice


Case 4: Colon Cancer Model


Human colorectal adenocarcinoma HT-29 cells were inoculated subcutaneously in B-NDG mice to successfully establish a colorectal adenocarcinoma model.


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A. Tumor growth curve, B. Body weight change of tumor-bearing mice


Available CDX models


Bone marrow

KG-1, K-562, NCI-H929

Brain

U-87 MG, LN-229

Breast

MDA-MB-231, DU4475, BT-474, MCF-7

Colorectal

HT-29, LOVO, HCT-8, LS 174T, HCT 116, COLO 205

Kidney

5637, G401

Liver

Hep G2, PLC/PRF/5, Hep 382.1-7, HuH-7

Lung

NCI-H520, A549, NCI-H1975, HCC827, NCI-H460

Ovary

SK-OV-3

Pancreas

PANC-1, MIA PaCa-2, BxPC-3

Prostate

Du 145, LNCaP clone FGC

SKIN

A-431, A375, A2058

Stomach

NCI-N87, SNU-5, NUGC-4, Hs 746T

Peripheral blood

RPMI 8226, Kasumi-1, HL-60, MV-4-11

Lymphoma

THP-1, Raji, NAMALWA, SU-DHL-1, Daudi


CDX Models Using Luciferase-Expressing Cell Lines


Cell  LineName

Disease

B-luc-GFP Raji

Burkitt's lymphoma

B-luc-GFP HT-29

Colorectal adenocarcinoma

B-luc-GFP NCI-H1975

Lung carcinoma, non-small cell (NSCLC)

B-luc K-562

Chronic myelogenous leukemia (CML)

B-luc MIA PaCa-2

Pancreatic carcinoma

B-luc B16-F10

Melanoma

B-luc EL4

Lymphoma

B-luc Hep 3B plus

Hepatocellular carcinoma (HCC)

B-luc Daudi

Lymphoma

B-Luc A20

Reticulum cell sarcoma


REFERENCES:


【1】Dranoff, G. Experimental mouse tumour models: what can be learnt about human cancer immunology? Nat Rev Immunol 12, 61-66 (2011).

【2】Kohnken, R., Porcu, P. & Mishra, A. Overview of the Use of Murine Models in Leukemia and Lymphoma Research. Front Oncol 7, 22 (2017).