top
Please input keywords
Order
*Country
한국
미국
일본
중국
영국
호주
프랑스
독일
이탈리아
싱가포르
인도
러시아
캐나다
스위스
기타 국가
*Province
*City
*Name
*Telephone
*Company
*Position
*Email
*Verification code
*Verification Code
B-hADGRG2 mice
Strain Name
C57BL/6-Adgrg2tm1(ADGRG2)Bcgen/Bcgen
Common Name  B-hADGRG2 mice
Background C57BL/6 Catalog number 112431
Aliases 
ADGRG2; Me6, Gpr64, B830041D06Rik
Description

  • ADGRG2 is a member of the G protein-coupled receptor family and is an epididymis specific transmembrane proteinGPR64 may be involved in a signal transduction pathway controlling epididymal function and male fertility. 
  • The exons 3~29 of mouse Adgrg2 gene that encodes the full-length protein was replaced by human ADGRG2 exons 3~28 in B-hADGRG2 mice.
  • ADGRG2 was detected in testis in both wild-type and homozygous B-hADGRG2 mice, as anti-ADGRG2 antibody was cross-reactive between human and mouse.

Protein expression analysis

from clipboard


Western blot analysis of ADGRG2 protein expression in homozygous B-hADGRG2 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+)  and homozygous B-hADGRG2 mice (H/H), and then analyzed by western blot with anti-ADGRG2 antibody. 40 μg total proteins were loaded for western blotting analysis. ADGRG2 was detected in testis in both wild-type and homozygous B-hADGRG2 mice, as anti-ADGRG2 antibody was cross-reactive between human and mouse.

mRNA expression analysis

from clipboard

Strain specific analysis of ADGRG2 mRNA expression in wild-type C57BL/6 mice and B-hADGRG2 mice by RT-PCR. Testis RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hADGRG2 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human ADGRG2 primers. Mouse ADGRG2 mRNA were detectable only in wild-type C57BL/6 mice. Human ADGRG2 mRNA was detectable only in homozygous B-hADGRG2 mice but not in wild-type mice.