| Strain Name |
C57BL/6-C5ar1tm1(C5AR1)Bcgen/Bcgen
|
Common Name | B-hC5AR1 mice |
| Background | C57BL/6 | Catalog number | 110078 |
|
Aliases |
C5A; C5AR; C5R1; CD88 | ||
|
NCBI Gene ID |
12273 | ||
mRNA expression analysis
Strain specific analysis of C5AR1 gene expression in wild-type C57BL/6 mice and homozygous B-hC5AR1 mice by RT-PCR. Mouse C5ar1 mRNA was detectable in lung tissue of wild-type C57BL/6 mice (+/+). Human C5AR1 mRNA was detectable only in homozygous B-hC5AR1 mice (H/H) but not in wild-type mice.
Protein expression analysis（Bone marrow)
Strain specific C5AR1 expression analysis in homozygous B-hC5AR1 mice by flow cytometry. Bone marrow were collected from WT and homozygous B-hC5AR1 (H/H) mice, and analyzed by flow cytometry with species-specific anti-C5AR1 antibody. Mouse C5AR1 was detectable in WT mice. Human C5AR1 was exclusively detectable in homozygous B-hC5AR1 dendritic cells but not WT mice.
Protein expression analysis（MC38 cell line & MC38 intratumora）
Strain specific C5AR1 expression analysis in MC38 cell line and MC38 intratumora by flow cytometry. MC38 cells were subcutaneously inoculated into C57BL/6 mice, and tumors were removed and prepare tumor cells when tumor volume reached appropriate size. MC38 cells and MC38 intratumora cells were collected and analyzed by flow cytometry with species-specific anti-C5AR1 antibody. Mouse C5AR1 was detectable in intratumora CD45+ cells but not in MC38 cells and intratumora CD45- cells.
Protein expression analysis（MC38 cell line & MC38 intratumora）
Strain specific C5a expression analysis in MC38 cell culture supernatant and MC38 tumor tissue grinding solution by flow ELISA. MC38 cells were subcutaneously inoculated into C57BL/6 mice, tumors were removed and prepare when tumor volume reached appropriate size. MC38 cell culture supernatant and MC38 tumor tissue grinding solution were collected and analyzed by ELISA with mice complement C5a ELISA kit. Mouse C5A was detectable in tumor tissus suspension but not in MC38 cell culture supernatant.
Protein expression analysis（Pan02 cell line & Pan02 intratumora）
Strain specific C5AR1 expression analysis in Pan02 intratumora by flow cytometry. Pan02 cells were subcutaneously inoculated into C57BL/6 mice, and tumors were removed and prepare tumor cells when tumor volume reached appropriate size. Pan02 intratumora cells were collected and analyzed by flow cytometry with species-specific anti-C5AR1 antibody. Mouse C5AR1 was detectable in intratumora mCD11b+mGr-1+ cells and mCD11b+mGr-1+mLy-6G+ cells.
Protein expression analysis（Pan02 cell line & Pan02 intratumora）
Strain specific C5a expression analysis in Pan02 cells, MC38 cells culture supernatant and Pan02, MC38 tumor tissue grinding solution by ELISA. Pan02 and MC38 cells were subcutaneously inoculated into C57BL/6 mice, tumors were removed and prepare when tumor volume reached appropriate size. Pan02 cells, MC38 cells culture supernatant and Pan02, MC38 tumor tissue grinding solution were collected and analyzed by ELISA with mice complement C5a ELISA kit. Mouse C5A was detectable in tumor tissus suspension but not in cell culture supernatant.
Analysis of leukocytes cell subpopulation in spleen
