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B-hCCR4 mice
Strain Name
C57BL/6-Ccr4tm1(CCR4)Bcgen/Bcgen
Common Name  B-hCCR4 mice
Background C57BL/6 Catalog number  170164
Related Genes 
CC-CKR-4, CD194, CKR4, CMKBR4, ChemR13, HGCN:14099, K5-5
NCBI Gene ID
12773

mRNA expression analysis

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Strain specific analysis of CCR4 gene expression in WT and hCCR4 mice by RT-PCR. Mouse Ccr4 mRNA was detectable only in splenocytes of wild-type (+/+) mice. Human CCR4 mRNA was detectable only in H/H, but not in +/+ mice. 


Protein expression analysis in CD4+ T cells


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Strain specific CCR4 expression analysis in homozygous B-hCCR4 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCCR4 (H/H) mice, and analyzed by flow cytometry with species-specific anti-CCR4 antibody. Mouse CCR4 was detectable in WT mice. Human CCR4 was exclusively detectable in homozygous B-hCCR4 but not WT mice.



Protein expression analysis in Tregs

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Strain specific CCR4 expression analysis in homozygous B-hCCR4 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCCR4 (H/H) mice, and analyzed by flow cytometry with species-specific anti-CCR4 antibody. Mouse CCR4 was detectable in WT mice. Human CCR4 was exclusively detectable in homozygous B-hCCR4 but not WT mice.


B-CAG-hCCR4 MC38 bind anti-human CCR4 antibody


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Analysis of B-CAG-hCCR4 MC38 by FACS. Flow cytometry analysis of the B-CAG-hCCR4 MC38 was performed to assess anti-human CCR4 Ab binding. Single live cells were gated for CD45 population and used for further analysis as indicated here. B-CAG-hCCR4 MC38 can bind well to anti-hCCR4 antibody (mogamulizumab, in house) vs isotype control. 

T cells in B-hCCR4 mice bind anti-human CCR4 antibody

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Analysis of splenocytes of B-hCCR4 mice by FACS. Splenocytes were isolated from female B-hCCR4 mice (6 week-old).  Flow cytometry analysis of the splenocytes was performed to assess anti-human CCR4 Ab binding with splenocytes. Single live cells were gated for CD45 population and used for further analysis as indicated here. T cells of the spleen in homozygous B-hCCR4 mice can bind well to anti-hCCR4 antibody (mogamulizumab, in house) vs isotype control. 

Tregs in B-hCCR4 mice bind anti-human CCR4 antibody

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Analysis of splenocytes of B-hCCR4 mice by FACS. Splenocytes were isolated from female B-hCCR4 mice (6 week-old).  Flow cytometry analysis of the splenocytes was performed to assess anti-human CCR4 Ab binding with Splenocytes. Single live cells were gated for CD45 population and used for further analysis as indicated here. Tregs of the spleen in homozygous B-hCCR4 mice can bind well to anti-hCCR4 antibody (mogamulizumab, in house) vs isotype control.