Strain Name |
C57BL/6N-Calcatm1(CALCA)Bcgen/Bcgen |
Common Name | B-hCGRP mice |
Background | C57BL/6N | Catalog number | 110118 |
Related Genes |
CALCA; CT; KC; PCT; CALC1; CGRP1; CGRP-I; CGRP-alpha |
mRNA and protein expression analysis
Strain specific analysis of CGRP gene expression in wild-type C57BL/6 mice and B-hCGRP mice by RT-PCR and western blot. (A) mRNA expression. Due to the high homology, sequencing were performed to verify whether humanization is successful (right). Combining the results of RT-PCR and sequencing, mouse Cgrp mRNA was detectable only in brain of wild-type C57BL/6 mice (+/+). Human CGRP mRNA was detectable only in homozygous B-hCGRP mice (H/H), but not in wild-type C57BL/6 mice. (B) Protein expression. Heart was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCGRP mice (H/H), and analyzed by western blot with a known cross-reactive anti-CGRP antibody. The CGRP protein can be detected in wild-type C57BL/6 mice and homozygous B-hCGRP mice.