Strain Name
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C57BL/6N-Cmklr1tm1(CMKLR1)Bcgen/Bcgen
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Common Name
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B-hCMKLR1 mice
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Background
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C57BL/6N
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Catalog number
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112875
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Aliases
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CHEMERINR, ChemR23, DEZ, ERV1, RVER1
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mRNA expression analysis
Strain specific analysis of CMKLR1 mRNA expression in wild-type C57BL/6 mice and B-hCMKLR1 mice by RT-PCR. Spleen RNA was isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hCMKLR1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human CMKLR1 primers. Mouse Cmklr1 mRNA was detectable in wild-type C57BL/6 mice. Human CMKLR1 mRNA was detectable only in homozygous B-hCMKLR1 mice but not in wild-type mice. Specially, the human CMKLR1 isoform b was actually transcribed in B-hCMKLR1 mice.
Protein expression analysis
Strain specific CMKLR1 expression analysis in homozygous B-hCMKLR1 mice by flow cytometry. Splenocytes, blood, lymph modes (LN) and bone marrow (BM) were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCMKLR1 mice (H/H), and analyzed by flow cytometry with species-specific anti-CMKLR1 antibody. mCMKLR1 was detectable in wild-type mice, hCMKLR1 was detectable in homozygous B-hCMKLR1 mice.
Strain specific CMKLR1 expression analysis in homozygous B-hCMKLR1 mice by flow cytometry. Splenocytes, blood and bone marrow (BM) were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCMKLR1 mice (H/H), and analyzed by flow cytometry with species-specific anti-CMKLR1 antibody. mCMKLR1 was detectable in wild-type mice, hCMKLR1 was detectable in homozygous B-hCMKLR1 mice.