Strain Name
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C57BL/6-Cxcr4tm1(CXCR4)Bcgen/Bcgen
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Common Name
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B-hCXCR4 mice
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Background
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C57BL/6
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Catalog number
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110100
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Related Genes
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CXC-R4, CXCR-4, CD184
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NCBI Gene ID
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12767
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mRNA expression analysis
Strain specific analysis of CXCR4 gene expression in WT and B-hCXCR4 mice by RT-PCR. Mouse Cxcr4 mRNA was detectable in splenocytes and thymocytes of wild-type (+/+). Human CXCR4 mRNA was detectable only in H/H, but not in +/+ mice.
Protein expression analysis in T cells
Strain specific CXCR4 expression analysis in homozygous B-hCXCR4 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCXCR4 (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific CXCR4 antibody. Mouse CXCR4 was detectable in WT mice. Human CXCR4 was exclusively detectable in homozygous B-hCXCR4, but the expression level was significantly lower than mouse CXCR4 in the C57BL/6 mice.
Protein expression analysis in CD4+ T cells
Strain specific CXCR4 expression analysis in homozygous B-hCXCR4 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCXCR4 (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific CXCR4 antibody. Mouse CXCR4 was detectable in WT mice. Human CXCR4 was exclusively detectable in homozygous B-hCXCR4, but the expression level was significantly lower than mouse CXCR4 in the C57BL/6 mice.
Protein expression analysis in B cells
Strain specific CXCR4 expression analysis in homozygous B-hCXCR4 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCXCR4 (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific CXCR4 antibody. Mouse CXCR4 was detectable in WT mice. Human CXCR4 was exclusively detectable in homozygous B-hCXCR4, but the expression level was significantly lower than mouse CXCR4 in the C57BL/6 mice.