Strain Name
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C57BL/6-Giprtm2(GIPR)Bcgen/Bcgen
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Common Name
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B-hGIPR mice
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Background
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C57BL/6
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Catalog number
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112714
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Aliases
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PGQTL2
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mRNA expression analysis
Strain specific analysis of GIPR mRNA expression in wild-type C57BL/6 mice and B-hGIPR mice by RT-PCR. Brain, eWAT and ingWAT RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hGIPR mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human GIPR primers. Mouse Gipr mRNA was detectable only in wild-type C57BL/6 mice. Human GIPR mRNA was detectable only in homozygous B-hGIPR mice but not in wild-type mice. eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue.
GIPR expression analysis by WB
Western blot analysis of GIPR protein expression in homozygous B-hGIPR mice. Various tissue lysates were collected from wild-type C57BL/6N mice (+/+) and homozygous B-hGIPR mice (H/H), and then analyzed by western blot with anti-GIPR antibody. 40 μg total proteins were loaded for western blotting analysis. GIPR was detected in brain, stomach, pancreas and liver.
GIPR expression analysis by IHC
Representative GIPR expression in different tissues of B-hGIPR mice by IHC. Tissues were stained with antibodies that recognized with both human and mouse GIPR (B-J) or anti-IgG antibody (A). Pancreas and brain of B-hGIPR mice and wild-type C57BL/6 mice show GIPR positive. Liver and adipose of B-hGIPR mice and wild-type C57BL/6 mice show GIPR negative. Mouse tissues from the wild-type C57BL/6 mice (A-E). Human pancreas carcinoma as a positive control (F). Other tissues were got from homozygous B-hGIPR mice (G-J). Original magnification ×200. Abbreviations: IHC, immunohistochemistry.
In vivo function of m/hGIP during IGPTT
In vivo function of m/hGIP during IGPTT. Plasma glucose and insulin concentrations were measured after intraperitoneal injection of 50 nmol/kg mouse GIP (Cat. HY-P77948, MCE) or human GIP (Cat. HY-P0276, MCE) and glucose (40%) in wild-type C57BL/6J mice and B-hGIPR mice. (male, 6-week-old, n=6). Data represent means ± SEM. Analyzed by 2way-ANOVA,*P<0.05, **P<0.01, ***P<0.001