Strain specific analysis of GPR55 mRNA expression in wild-type C57BL/6 mice and B-hGPR55 mice by RT-PCR. Brain RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hGPR55 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human GPR55 primers. Mouse Gpr55 mRNA was detectable only in wild-type C57BL/6 mice. Human GPR55 mRNA was detectable only in homozygous B-hGPR55 mice but not in wild-type mice.

Western blot analysis of GPR55 protein expression in homozygous B-hGPR55 mice. Various tissue lysates were collected from C57BL/6N wildtype mice (+/+) and homozygous B-hGPR55 mice (H/H), and then analyzed by western blot with anti-GPR55 antibody. 40 μg total proteins were loaded for western blotting analysis. GPR55 was detected in brain, pancreas, liver and stomach in homozygous B-hGPR55 mice. （The arrow above indicates the glycosylated GPR55 protein, the arrows below indicates the GPR55 protein）