Strain Name
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C57BL/6N-Gpr84tm1(GPR84)Bcgen/Bcgen
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Common Name
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B-hGPR84 mice
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Background
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C57BL/6N
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Catalog number
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111211
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Aliases
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EX33, GPCR4
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mRNA expression analysis
Strain specific analysis of GPR84 mRNA expression in wild-type C57BL/6N mice, B-hGPR84 mice and B-GPR84 KO mice by RT-PCR. Brain RNA were isolated from wild-type C57BL/6N mice (+/+), homozygous B-hGPR84 mice (H/H) and B-GPR84 KO mice (Δ/Δ), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human GPR84 primers. Mouse Gpr84 mRNA was detectable only in wild-type C57BL/6N mice. Human GPR84 mRNA was detectable only in homozygous B-hGPR84 mice.
Protein expression analysis
Western blot analysis of GPR84 protein expression in homozygous B-hGPR84 mice. Various tissue lysates were collected from wild-type C57BL/6N mice (+/+) and homozygous B-hGPR84 mice (H/H), and then analyzed by western blot with anti-GPR84 antibody. (anti-human GPR84 antibody: Santa Cruz, sc-293447). 40 μg total proteins were loaded for western blotting analysis. GPR84 was detected in lung, liver, heart and bone marrow, as the antibody is cross-recognized between human and mouse GPR84. (There was no β-actin in the heart tissue, there was no GAPDH in the bone marrow tissue.)