top
Please input keywords
Order
*Country
한국
미국
일본
중국
영국
호주
프랑스
독일
이탈리아
싱가포르
인도
러시아
캐나다
스위스
기타 국가
*Province
*City
*Name
*Telephone
*Company
*Position
*Email
*Verification code
*Verification Code
B-hGPR84 mice
Strain Name C57BL/6N-Gpr84tm1(GPR84)Bcgen/Bcgen Common Name  B-hGPR84 mice
Background C57BL/6N Catalog number 111211
Aliases 
EX33, GPCR4
mRNA expression analysis

from clipboard

Strain specific analysis of GPR84 mRNA expression in wild-type C57BL/6N mice, B-hGPR84 mice and B-GPR84 KO mice by RT-PCR. Brain RNA were isolated from wild-type C57BL/6N mice (+/+), homozygous B-hGPR84 mice (H/H) and B-GPR84 KO mice (Δ/Δ), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human GPR84 primers. Mouse Gpr84 mRNA was detectable only in wild-type C57BL/6N mice. Human GPR84 mRNA was detectable only in homozygous B-hGPR84 mice. 

Protein expression analysis

from clipboard

Western blot analysis of GPR84 protein expression in homozygous B-hGPR84 mice. Various tissue lysates were collected from wild-type C57BL/6N mice (+/+) and homozygous B-hGPR84 mice (H/H), and then analyzed by western blot with anti-GPR84 antibody. (anti-human GPR84 antibody: Santa Cruz, sc-293447). 40 μg total proteins were loaded for western blotting analysis. GPR84 was detected in lung, liver, heart and bone marrow, as the antibody is cross-recognized between human and mouse GPR84. (There was no β-actin in the heart tissue, there was no GAPDH in the bone marrow tissue.)