Strain Name
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C57BL/6N-Gpr87tm1(GPR87)Bcgen/Bcgen
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Common Name
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B-hGPR87 mice
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Background
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C57BL/6N
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Catalog number
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112529
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Aliases
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FKSG78, GPR95, KPG_002
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mRNA expression analysis
Strain specific analysis of GPR87 mRNA expression in wild-type C57BL/6 mice and B-hGPR87 mice by RT-PCR. Lung RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hGPR87 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human GPR87 primers. Mouse Gpr87 mRNA was detectable only in wild-type C57BL/6 mice. Human GPR87 mRNA was detectable only in homozygous B-hGPR87 mice but not in wild-type mice.
Protein expression analysis
Western blot analysis of GPR87 protein expression in homozygous B-hGPR87 mice. Various tissue lysates were collected from C57BL/6N wildtype (+/+) mice and homozygous B-hGPR87 (H/H) mice, and then analyzed by western blot with anti-GPR87 antibody. 40 μg total proteins were loaded for western blotting analysis. GPR87 was detected in stomach, lung, esophagus and bladder.