B-hGPRC5D mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

Strain Name	C57BL/6N-Gprc5d^{tm1(GPRC5D)Bcgen}/Bcgen	Common Name	B-hGPRC5D mice
Background	C57BL/6N	Catalog number	111240
Related Genes	/

mRNA expression analysis

Strain specific analysis of GPRC5D gene expression in wild-type C57BL/6 mice and B-hGPRC5D mice by RT-PCR. Mouse Gprc5d mRNA was detectable only in testis of wild-type C57BL/6 mice (+/+). Human GPRC5D mRNA was detectable only in homozygous B-hGPRC5D mice (H/H), but not in wild-type mice.

Protein expression analysis

Strain specific GPRC5D expression analysis in homozygous B-hGPRC5D mice by western blot.

Spleen was collected from WT mice (+/+) and homozygous B-hGPRC5D (H/H) mice, and analyzed by western blot with anti-GPRC5D antibody. GPRC5D was detectable in WT mice (+/+) and homozygous B-hGPRC5D mice (H/H) due to the cross-reactivity of antibodies.