Strain Name
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C57BL/6N-Hcar1tm1(HCAR1)Bcgen/Bcgen
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Common Name
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B-hHCAR1 mice
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Background
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C57BL/6N
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Catalog number
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111919
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Aliases
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HCAR1 (FKSG80, GPR104, GPR81, HCA1, LACR1, TA-GPCR, TAGPCR)
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mRNA expression analysis
Strain specific analysis of HCAR1 gene expression in wild-type C57BL/6 mice and B-hHCAR1 mice by RT-PCR. Mouse Hcar1 mRNA was detectable only in BAT (brown adipose tissue) of wild-type C57BL/6 mice (+/+). Human HCAR1 mRNA was detectable only in homozygous B-hHCAR1 mice (H/H), but not in wild-type mice.
Protein expression analysis
Strain specific HCAR1 expression analysis in homozygous B-hHCAR1 mice by flow cytometry. BAT (brown adipose tissue) was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hHCAR1 mice (H/H), and analyzed by western blot with anti-HCAR1 antibody. HCAR1 was both detectable in wild-type mice and homozygous B-hHCAR1 mice (H/H) due to the cross-reactivity of antibodies.