Strain Nam
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C57BL/6N-Mc4rtm1(MC4R)Bcgen/Bcgen
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Common Name
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B-hMC4R mice
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Background
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C57BL/6N
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Catalog number
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112592
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Aliases
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MC4R: BMIQ20
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mRNA expression analysis
Strain specific analysis of MC4R mRNA expression in wild-type C57BL/6N mice and B-hMC4R mice by RT-PCR. Brain RNA were isolated from wild-type C57BL/6N mice (+/+) and homozygous B-hMC4R mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human MC4R primers. Mouse Mc4r mRNA was detectable only in wild-type C57BL/6N mice. Human MC4R mRNA was detectable only in homozygous B-hMC4R mice but not in wild-type mice.