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B-hRSPO3/hLGR5 mice
Strain Name
C57BL/6-Rspo3tm1(RSPO3)Bcgen Lgr5tm1(LGR5)Bcgen/Bcgen
Common Name  B-hRSPO3/hLGR5 mice
Background C57BL/6 Catalog number 110857
Aliases 

CRISTIN1, PWTSR, THSD2; FEX, GPR49, GPR67, GRP49, HG38

mRNA expression analysis 

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Strain specific analysis of LGR5 mRNA expression in wild-type C57BL/6 mice and B-hRSPO3/hLGR5 mice by RT-PCR. Stomach RNA was isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hRSPO3hLGR5 mice (H/H; H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human LGR5 primers. Mouse Lgr5 mRNA was detectable only in wild-type C57BL/6 mice. Human LGR5 mRNA was detectable only in homozygous B-hRSPO3/hLGR5 mice but not in wild-type mice. 

Strain specific analysis of RSPO3 mRNA expression in wild-type C57BL/6 mice and B-hRSPO3/hLGR5 mice by RT-PCR. Stomach RNA was isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hRSPO3hLGR5 mice (H/H; H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human RSPO3 primers. Mouse Rspo3 mRNA was detectable only in wild-type C57BL/6 mice. Human RSPO3 mRNA was detectable only in homozygous B-hRSPO3/hLGR5 mice but not in wild-type mice. 

Protein expression analysis

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Western blot analysis of LGR5 protein expression in homozygous B-hRSPO3/hLGR5 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hRSPO3/hLGR5 mice (H/H; H/H), and then analyzed by western blot with anti-LGR5 antibody. 40 μg total proteins were loaded for western blotting analysis. LGR5 was detected in stomach, colon, frontal lobe, kidney, liver and lung.

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Western blot analysis of RSPO3 protein expression in homozygous B-hRSPO3/hLGR5 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hRSPO3/hLGR5 mice (H/H; H/H), and then analyzed by western blot with anti-RSPO3 antibody. 40 μg total proteins were loaded for western blotting analysis. RSPO3 was detected in stomach.