B-hIL36A mice
| Strain Name |
C57BL/6N-Il36atm1(IL36A)Bcgen/Bcgen
|
Common Name | B-hIL36A mice |
| Background | C57BL/6N | Catalog number | 110092 |
|
Aliases |
IL36A, FIL1, FIL1(EPSILON), FIL1E, IL-1F6, IL1(EPSILON), IL1F6, interleukin 36 alpha | ||
|
NCBI Gene ID |
54448 | ||
Targeting strategy
Gene targeting strategy for B-hIL36A mice.
The exon 2-5 of mouse Il36a gene that encodes the full-length protein were replaced by human IL36A exon 1-4 in B-hIL36A mice.
mRNA expression analysis
Strain specific analysis of IL36A gene expression in wild type (WT) mice and B-hIL36A mice by RT-PCR.
Mouse Il36a mRNA was detectable only in stomach of WT mice (+/+). Human IL36A mRNA was detectable only in homozygous B-hIL36A mice (H/H) but not in WT mice (+/+).
Protein expression analysis
Strain specific IL36A expression analysis in homozygous B-hIL36A mice by western blot .
Skin were collected from wild type (WT) mice (+/+) and homozygous B-hIL36A mice (H/H), and analyzed by western blot with anti-IL36A antibody. Mouse IL36A was detectable in WT mice (+/+) due to the cross-reactivity of antibodies. Human IL36A was detectable in homozygous B-hIL36A mice (H/H) but not in WT mice (+/+).
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