top
Please input keywords
Order
*Country
한국
미국
일본
중국
영국
호주
프랑스
독일
이탈리아
싱가포르
인도
러시아
캐나다
스위스
기타 국가
*Province
*City
*Name
*Telephone
*Company
*Position
*Email
*Verification code
*Verification Code
B-hIL36A mice
Strain Name
C57BL/6N-Il36atm1(IL36A)Bcgen/Bcgen 
Common Name  B-hIL36A mice
Background C57BL/6N Catalog number  110092
Aliases 
IL36A, FIL1, FIL1(EPSILON), FIL1E, IL-1F6, IL1(EPSILON), IL1F6, interleukin 36 alpha
NCBI Gene ID
54448
Targeting strategy
Gene targeting strategy for B-hIL36A mice. 
The exon 2-5 of mouse Il36a gene that encodes the full-length protein were replaced by human IL36A exon 1-4 in B-hIL36A mice.


mRNA expression analysis

from clipboard

Strain specific analysis of IL36A gene expression in wild type (WT) mice and B-hIL36A mice by RT-PCR. 

Mouse Il36a mRNA was detectable only in stomach of WT mice (+/+). Human IL36A mRNA was detectable only in homozygous B-hIL36A mice (H/H) but not in WT mice (+/+). 


Protein expression analysis

from clipboard

Strain specific IL36A expression analysis in homozygous B-hIL36A mice by western blot . 
Skin were collected from wild type (WT) mice (+/+) and homozygous B-hIL36A mice (H/H), and analyzed by western blot with anti-IL36A antibody. Mouse IL36A was detectable in WT mice (+/+) due to the cross-reactivity of antibodies. Human IL36A was detectable in homozygous B-hIL36A mice (H/H) but not in WT mice (+/+).