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B-hADM mice
Strain Name
C57BL/6N-Admtm1(ADM)Bcgen/Bcgen 
Common Name  B-hADM mice
Background C57BL/6N Catalog number 111150
Aliases 

AM; PAMP

Protein expression analysis

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Strain specific ADM expression analysis in homozygous B-hADM mice by ELISA. Plasma was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hADM mice (H/H) and analyzed by ELISA with ADM ELISA kit. ADM was detectable in wild-type mice and homozygous B-hADM mice as the kit is crossly reactive with ADM in human and mice.

mRNA expression analysis

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Strain specific analysis of ADM mRNA expression in wild-type C57BL/6 mice and B-hADM mice by RT-PCR. Kidney RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hADM mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human ADM primers. Mouse Adm mRNA was detectable only in wild-type C57BL/6 mice. Human ADM mRNA was detectable only in homozygous B-hADM mice but not in wild-type mice.