mRNA expression analysis

Strain specific analysis of  ANGPTL8 gene expression in wild-type mice and B-hANGPTL8 mice by RT-PCR. Mouse Angptl8 mRNA was detectable in the wild-type mice (+/+). Human ANGPTL8 mRNA was detectable only in homozygous B-hANGPTL8 mice but not in wild-type mice.

Protein expression analysis 

Strain specific ANGPTL8 expression analysis in homozygous B-hANGPTL8 mice by ELISA. 
Plasma was collected from wild-type mice (+/+) and homozygous B-hANGPTL8 mice (H/H), and analyzed by ELISA. ANGPTL8 was both detectable in wild-type mice and B-hANGPTL8 mice (H/H) due to the cross-reactivity of ELISA kit.