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B-hCCL3 mice
Strain Name C57BL/6N-Ccl3tm1(CCL3)Bcgen/Bcgen Common Name  B-hCCL3 mice
Background C57BL/6N Catalog number 111439
Related Genes 

CCL3 (G0S19-1, LD78ALPHA, MIP-1-alpha, MIP1A, SCYA3)

mRNA expression analysis

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Strain specific analysis of CCL3 gene expression in wild-type mice and B-hCCL3 mice by RT-PCR. Mouse Ccl3 mRNA was detectable in splenocytes of wild-type mice (+/+). Human CCL3 mRNA was detectable only in homozygous B-hCCL3 mice (H/H) but not in wild-type mice.

Protein expression analysis

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Strain specific CCL3 expression analysis in homozygous B-hCCL3 mice by ELISA. Bone marrow-derived macrophages(BMDM) were isolated from wild-type mice (+/+) and homozygous B-hCCL3 mice (H/H) and stimulated with 10ng/ml LPS in vitro, cell culture supernates were analyzed by ELISA with species-specific CCL3 ELISA kit. Mouse CCL3 was detectable in wild-type mice. Human CCL3 was exclusively detectable in homozygous B-hCCL3 mice but not in wild-type mice.