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B-hCD59 mice
Strain Name
C57BL/6-Cd59b,Cd59atm1(CD59)Bcgen/Bcgen
Common Name  B-hCD59 mice
Background C57BL/6 Catalog number 111427
Aliases 

16.3A5, 1F5, EJ16, EJ30, EL32, G344, HRF-20, HRF20, MAC-IP, 

MACIF, MEM43, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18-20

Protein expression analysis of CD59 in T cells and B cells

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Mouse CD59a and human CD59 expression analysis in T cells and B cells. T cells and B cells were isolated from the blood of wild type C57BL/6 mice, homozygous B-hCD59 mice and human blood cells. CD59 expressed on T cells and B cells were analyzed by flow cytometry with anti-CD59 antibody. Mouse CD59a were detectable on T cells and B cells of wild type C57BL/6, but not on the cells of B-hCD59 mice and human blood cells. Human CD59 was detectable on T cells and B cells of B-hCD59 mice and human blood cells, but not on the cells of wild type C57BL/6 mice.

Protein expression analysis of CD59 in NK cells, dendritic cells and macrophages

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Mouse CD59a and human CD59 expression analysis in NK, dendritic cells and macrophages. NK cells, dendritic cells and macrophages were isolated from blood of wild type C57BL/6 mice and homozygous B-hCD59 mice. CD59 expressed on NK cells, dendritic cells and macrophages were analyzed by flow cytometry with anti-CD59 antibody. Mouse CD59a were detectable on NK cells, dendritic cells and macrophages of wild type C57BL/6, but not on the cells of B-hCD59 mice. Human CD59 was exclusively detectable on NK cells, dendritic cells and macrophages of B-hCD59 mice.

Protein expression analysis of CD59 in monocytes and granulocytes

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Mouse CD59a and human CD59 expression analysis in monocytes and granulocytes. Monocytes and granulocytes were isolated from the blood of wild type C57BL/6 mice,  homozygous B-hCD59 mice and human blood cells. CD59 expressed on monocytes and granulocytes were analyzed by flow cytometry with anti-CD59 antibody. Mouse CD59a was detectable on monocytes and granulocytes of wild type C57BL/6 mice, but not on the cells of B-hCD59 mice and human blood cells. Human CD59 was detectable on monocytes and granulocytes of B-hCD59 mice and human blood cells, but not on the cells of wild type C57BL/6 mice.

Protein expression analysis of CD59 in platelets and red blood cells 

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Mouse CD59a and human CD59 expression analysis in platelets and red blood cells . Platelets and red blood cells were isolated from the blood of wild type C57BL/6 mice,   homozygous B-hCD59 mice and human blood cells. CD59 expressed on platelets and red blood were analyzed by flow cytometry with anti-CD59 antibody. Mouse CD59a was detectable on platelets and red blood cells of wild type C57BL/6 mice, but not on the cells of B-hCD59 mice and human blood cells. Human CD59 was detectable on platelets and red blood cells of B-hCD59 mice and human blood cells, but not on the cells of wild type C57BL/6 mice.