B-hGDF15 mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

Strain Name	C57BL/6N-Gdf15^{tm1(GDF15)Bcgen}/Bcgen	Common Name	B-hGDF15 mice
Background	C57BL/6N	Catalog number	111201
Related Genes	growth differentiation factor 15, GDF-15, MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB
NCBI Gene ID	23886

mRNA expression analysis

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Strain specific analysis of GDF15 gene expression in wild-type mice and B-hGDF15 mice by RT-PCR. Mouse Gdf15 mRNA was detectable in kidney of wild-type (+/+). Human GDF15 mRNA was detectable only in H/H but not in +/+ mice.

Protein expression analysis

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Strain specific GDF15 expression analysis in homozygous B-hGDF15 mice by ELISA. Serum was collected from wild-type mice (+/+) and homozygous B-hGDF15 mice (H/H) analyzed by ELISA with species-specific GDF15 ELISA kit. Mouse GDF15 was exclusively detectable in wild-type mice and human GDF15 was exclusively detectable in homozygous B-hGDF15 mice. ND: Not detected.