B-hGFRAL mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

Strain Name	C57BL/6-Gfral^{tm1(GFRAL)Bcgen}/Bcgen	Common Name	B-hGFRAL mice
Background	C57BL/6	Catalog number	111202
Related Genes	GDNF family receptor alpha like, C6orf144, GRAL, UNQ9356, bA360D14.1

mRNA expression analysis

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Strain specific analysis of GFRAL gene expression in wild-type mice and B-hGFRAL mice by RT-PCR. Mouse Gfral mRNA was detectable in brain of wild-type mice (+/+). Human GFRAL mRNA was detectable only in brain of homozygous B-hGFRAL mice but not in wild-type mice.

Protein expression analysis

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Strain specific analysis of GFRAL expression in C57BL/6(+/+) and homozygous B-hGFRAL(H/H) mice by western blot. Ovary and stomach were collected from C57BL/6(+/+) and homozygous B-hGFRAL(H/H) mice. GFRAL was detectable in C57BL/6(+/+) and B-hGFRAL (H/H) mice due to the cross-reactivity of antibodies.