IFNG and IFNGR1 expression analysis in B-hIFNGR1/hIFNG mice by RT-PCR. Human IFNG and IFNGR1 mRNA were detectable in splenocytes of the homozygous B-hIFNGR1/hIFNG mice (H/H,H/H) but not in wild-type mice (+/+).

IFNG and IFNGR1 expression analysis in B-hIFNGR1/IFNG mice. (A) IFNG expression analysis. Serum was collected from wild-type mice (+/+) and homozygous B-hIFNGR1/hIFNG mice (H/H,H/H) after anti-CD3ε stimulated for 2 hours, and analyzed by ELISA with species-specific IFNG ELISA kit. Mouse IFNG was exclusively detectable in wild-type mice and human IFNG was exclusively detectable in homozygous B-hIFNGR1/hIFNG mice. (B) IFNGR1 expression analysis. Peritoneal derived macrophage cells were collected from wild-type and B-hIFNGR1/hIFNG mice, and analyzed by flow cytometry with anti-IFNGR1 antibody. Human IFNGR1 was detectable in B-hIFNGR1/hIFNG mice but not in wild-type mice.