B-hIL2/hIL2RA/hIL2RB/hIL2RG mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

Please input keywords

Order

*Country

한국

미국

일본

중국

영국

호주

프랑스

독일

이탈리아

싱가포르

인도

러시아

캐나다

스위스

기타 국가

*Province

*City

*Name

*Telephone

*Company

*Position

*Email

*Verification code

*Verification Code

Home

동물/세포 모델

동물/세포모델

Humanized Mouse Models

Cytokine Humanized Mice

Text

B-hIL2/hIL2RA/hIL2RB/hIL2RG mice

Strain Name

C57BL/6-Il2^{tm1(IL2)Bcgen}Il2ra^{tm1(IL2RA) Bcgen}ll2rb^{tm2(IL2RB)Bcgen}ll2rg^{tm2(IL2RG)Bcgen}/Bcgen

Common Name

B-hIL2/hIL2RA/hIL2RB/hIL2RG mice

Background

C57BL/6

Catalog number

112748

Aliases

IL2 also known as TCGF, lymphokine
IL2RA also known as CD25, IDDM10, IL2R, IMD41, TCGFR, p55
IL2RB also known as CD122.
IL2RG also known as CD132, SCIDX, IL-2RG

Protein expression analysis

from clipboard

Strain specific IL2 expression analysis in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice by ELISA. Serum were collected from wild-type mice C57BL/6 mice and homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice stimulated with anti-mCD3e antibody (7.5 μg/mice, i.p.) and anti-CD28 antibody (5 μg/mice, i.p.) in vivo for 2 h, and analyzed by ELISA with species-specific IL2 ELISA kit. (A) Mouse IL2 was only detectable in wild-type mice. (B) Human IL2 was exclusively detectable in homozygous mice but not in wild-type mice.

Mice used in the experiment: male, 6-week-old, n=3/group.

Protein expression analysis of IL2RA in T cells

from clipboard

Strain specific IL2RA expression analysis in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice and homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice stimulated with anti-CD3e antibody in vivo (7.5 μg/mice, i.p.), and analyzed by flow cytometry with species-specific anti-IL2RA antibody. mIL2RA was only detectable in wild-type mice, and hIL2RA was exclusively detectable in T cells in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice.

Mice used in the experiment: male, 8-week-old, n=3/group.

Protein expression analysis of IL2RA in CD4+ T cells and CD8+ T cells

from clipboard

Strain specific IL2RA expression analysis in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice and homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice stimulated with anti-CD3e antibody in vivo (7.5 μg/mice, i.p.), and analyzed by flow cytometry with species-specific anti-IL2RA antibody. mIL2RA was only detectable in wild-type mice, and hIL2RA was exclusively detectable in CD4+ T and CD8+ T cells in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice.

Mice used in the experiment: male, 8-week-old, n=3/group.

Protein expression analysis of IL2RB in T cells

from clipboard

Strain specific IL2RB expression analysis in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice and homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice stimulated with anti-CD3e antibody in vivo (7.5 μg/mice, i.p.), and analyzed by flow cytometry with species-specific anti-IL2RB antibody. mIL2RB was only detectable in wild-type mice, and hIL2RB was exclusively detectable in T cells in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice.

Mice used in the experiment: male, 8-week-old, n=3/group.

Protein expression analysis of IL2RB in CD4+ T cells and CD8+ T cells

from clipboard

Strain specific IL2RB expression analysis in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice and homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice stimulated with anti-CD3e antibody in vivo (7.5 μg/mice, i.p.), and analyzed by flow cytometry with species-specific anti-IL2RB antibody. mIL2RB was only detectable in wild-type mice, and hIL2RB was exclusively detectable in CD4+ T and CD8+ T cells in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice.

Mice used in the experiment: male, 8-week-old, n=3/group.

Protein expression analysis of IL2RG in T cells

from clipboard

Strain specific IL2RG expression analysis in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice and homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice, and cultured in 96-well plates pre-coated with 2 μg/mL anti-mCD3e and 5 μg/mL anti-mCD28 antibody for 24 h, and then cells were harvested and analyzed by flow cytometry with species-specific anti-IL2RG antibody. mIL2RG was only detectable in wild-type mice, while hIL2RG was exclusively detectable in T cells in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice.

Mice used in the experiment: male, 8-week-old, 1 mice/group.

Protein expression analysis of IL2RG in CD4+ T cells

from clipboard

Strain specific IL2RG expression analysis in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice and homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice, and cultured in 96-well plates pre-coated with 2 μg/mL anti-mCD3e and 5 μg/mL anti-mCD28 antibody for 24 h, and then cells were harvested and analyzed by flow cytometry with species-specific anti-IL2RG antibody. mIL2RG was only detectable in wild-type mice, while hIL2RG was exclusively detectable in CD4+ T cells in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice.

Mice used in the experiment: male, 8-week-old, 1 mice/group.

Protein expression analysis of IL2RG in CD8+ T cells

from clipboard

Strain specific IL2RG expression analysis in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice and homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice, and cultured in 96-well plates pre-coated with 2 μg/mL anti-mCD3e and 5 μg/mL anti-mCD28 antibody for 24 h, and then cells were harvested and analyzed by flow cytometry with species-specific anti-IL2RG antibody. mIL2RG was only detectable in wild-type mice, while hIL2RG was exclusively detectable in CD8+ T cells in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice.

Mice used in the experiment: male, 8-week-old, 1 mice/group.

Phosphorylation of STAT5 induced by IL2

Intracellular phosphorylation of pSTAT5 analysis in splenocytes by flow cytometry. Splenocytes were harvested and analyzed for pSTAT5 induction in the T cell subtypes of wild type C57BL/6 mice and B-hIL2/hIL2R mice. IL2 induced pSTAT5 in T cells with a dose dependent manner. Specifically induced high level of pSTAT5 in CD8+T cells. At 10ng/mL concentration, human IL2 induced higher pSTAT5 in T cells of B-hIL2/hIL2R mice than that in wild type C57BL/6 mice.

Analysis of leukocytes cell subpopulation in spleen

from clipboard

Frequency of leukocyte subpopulations in spleen by flow cytometry. Splenocytes were isolated from male wild-type C57BL/6 mice (n=3, 8-week-old) and homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice (n=3, 9-week-old). A. Flow cytometry analysis of the splenocytes was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, B cells, dendritic cells, neutrophils, macrophages, CD4+ T cells, CD8+ T cells and Tregs in B-hIL2/hIL2RA/IL2RB/IL2RG mice were similar to those in C57BL/6 mice. NK cells and monocytes decreased in humanized mice. Values are expressed as mean ± SD. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***p < 0.001.

Frequency of leukocyte subpopulations in blood

from clipboard

Frequency of leukocyte subpopulations in blood by flow cytometry. Blood cells were isolated from male wild-type C57BL/6 mice (n=3, 8-week-old) and homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice (n=3, 9-week-old). A. Flow cytometry analysis of the blood cells was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, B cells, dendritic cells, neutrophils, macrophages, CD4+ T cells, CD8+ T cells and Tregs in B-hIL2/hIL2RA/IL2RB/IL2RG mice were similar to those in C57BL/6 mice. NK cells and monocytes decreased in humanized mice. Values are expressed as mean ± SD. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***p < 0.001.

Frequency of leukocyte subpopulations in lymph nodes

from clipboard

Frequency of leukocyte subpopulations in lymph nodes by flow cytometry. Lymph nodes cells were isolated from male wild-type C57BL/6 mice (n=3, 8-week-old) and homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice (n=3, 9-week-old). A. Flow cytometry analysis of the lymph nodes cells was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, B cells, CD4+ T cells, CD8+ T cells and Tregs in B-hIL2/hIL2RA/IL2RB/IL2RG mice were similar to those in C57BL/6 mice. NK cells decreased in humanized mice. Values are expressed as mean ± SD. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***p < 0.001.

Frequency of leukocyte subpopulations in thymus

Frequency of leukocyte subpopulations in thymus by flow cytometry. Thymus cells were isolated from male wild-type C57BL/6 mice (n=3, 8-week-old) and homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice (n=3, 9-week-old). A. Flow cytometry analysis of the thymus cells was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, CD4+ T cells, CD8+ T cells and Tregs in B-hIL2/hIL2RA/IL2RB/IL2RG mice were similar to those in C57BL/6 mice. Values are expressed as mean ± SD. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***p < 0.001.