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B-hIL27 mice
Strain Name
C57BL/6-Il27tm1(IL27)Bcgen/Bcgen
Common Name  B-hIL27 mice
Background C57BL/6 Catalog number  110763
Related Genes 

IL-27, IL-27A, IL27A, IL27p28, IL30, MGC71873, p28

NCBI Gene ID
246779

Targeting strategy


Gene targeting strategy for B-hIL27 mice. The mouse Il27 gene was replaced by full coding region gene sequence of human IL27 in B-hIL27 mice.



mRNA expression analysis

from clipboard

Strain specific analysis of  IL27 gene expression in wild-type mice and B-hIL27 mice by RT-PCR. Mouse Il27 was detectable in the BMDCs of wild-type mice (+/+). Human IL27 was only detectable in homozygous B-hIL27 mice but not in wild-type mice.


Protein expression analysis

from clipboard

Strain specific IL27 expression analysis in homozygous B-hIL27 mice by ELISA. BMDC culture supernatant was collected from wild-type mice (+/+) and homozygous B-hIL27 mice (H/H) stimulated with LPS (1μg/mL) for 24h, and analyzed by ELISA with species-specific IL27 ELISA kit. Mouse IL27 was exclusively detectable in wild-type mice. Human IL27 was detectable in homozygous B-hIL27 mice but not in wild-type mice.