Targeting strategy

Gene targeting strategy for B-hIL27 mice. The mouse Il27 gene was replaced by full coding region gene sequence of human IL27 in B-hIL27 mice.

mRNA expression analysis

Strain specific analysis of  IL27 gene expression in wild-type mice and B-hIL27 mice by RT-PCR. Mouse Il27 was detectable in the BMDCs of wild-type mice (+/+). Human IL27 was only detectable in homozygous B-hIL27 mice but not in wild-type mice.

Protein expression analysis

Strain specific IL27 expression analysis in homozygous B-hIL27 mice by ELISA. BMDC culture supernatant was collected from wild-type mice (+/+) and homozygous B-hIL27 mice (H/H) stimulated with LPS (1μg/mL) for 24h, and analyzed by ELISA with species-specific IL27 ELISA kit. Mouse IL27 was exclusively detectable in wild-type mice. Human IL27 was detectable in homozygous B-hIL27 mice but not in wild-type mice.