Strain specific IL33 and TSLP expression analysis in homozygous B-hIL33/hTSLP/hTSLPR mice by ELISA. Calcipotriol (MC903) was dissolved in ethanol and topically applied on ears of either wild type C57BL/6 mice (+/+) or homozygous B-hIL33/hTSLP/hTSLPR mice (H/H) for 7 days. n=3. Ear grinding supernatant from the two mice was analyzed  by ELISA. Mouse IL33 and TSLP were only detectable in wild type C57BL/6 mice. Human IL33 and TSLP were exclusively detectable in homozygous B-hIL33/hTSLP/hTSLPR mice but not in wild type mice. ND: not detectable.

Strain specific TSLPR expression analysis in homozygous B-hIL33/hTSLP/hTSLPR mice by flow cytometry. Bone marrow was collected from wild type C57BL/6 mice (+/+) and homozygous B-hIL33/hTSLP/hTSLPR mice (H/H), Dendritic cells were induced from bone marrow cells in culture and analyzed by flow cytometry with species-specific anti-TSLPR antibody. Mouse TSLPR was only detectable in wild type C57BL/6 mice. Human TSLPR was only detectable in homozygous B-hIL33/hTSLP/hTSLPR mice but not in wild type mice.