Strain specific analysis of IL6ST gene expression in wild-type C57BL/6 mice and B-hIL6ST mice by RT-PCR. Mouse Il6st mRNA was detectable only in lung and bladder of wild-type C57BL/6 mice. Human IL6ST mRNA was detectable only in homozygous B-hIL6ST mice but not in wild-type mice. 

Strain specific IL6ST expression analysis in homozygous B-hIL6ST mice by ELISA. Serum was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hIL6ST mice (H/H) and analyzed by ELISA with species-specific IL6ST ELISA kit. Human IL6ST was exclusively detectable in homozygous B-hIL6ST mice but not in wild-type mice.