mRNA expression analysis

Strain specific analysis of IL9R gene expression in B-hIL9R mice by RT-PCR. Mouse Il9 was detectable in splenocytes of wild-type mice (+/+). Human IL9R was detectable only in homozygous B-hIL9R mice but not in wild-type mice.

Strain specific IL9R expression analysis in homozygous B-hIL9R mice by flow cytometry. Splenocytes were collected from wild-type mice (+/+) and homozygous B-hIL9R mice (H/H) stimulated with anti-CD40 in vitro (10 μg/mL), and analyzed by flow cytometry with species-specific anti-IL9R antibody. Mouse IL9R was detectable in wild-type mice. Human IL9R was exclusively detectable in homozygous B-hIL9R but not in wild-type mice.