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B-hKLK2/hKLK3 mice
Strain Name
C57BL/6-KLK2tm1(KLK2)Bcgen KLK3tm1(KLK3)Bcgen/Bcgen
Common Name  B-hKLK2/hKLK3 mice
Background C57BL/6 Catalog number 112233
Aliases 
KLK2: hK2, hGK-1, KLK2A2;
KLK3: APS, PSA, hK3, KLK2A1
mRNA expression analysis

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Strain specific analysis of KLK2 mRNA expression in wild-type C57BL/6 mice and B-hKLK2/hKLK3 mice by RT-PCR. Prostate RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hKLK2/hKLK3 mice (H/H;H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with human KLK2 primers. Human KLK2 mRNA was detectable only in homozygous B-hKLK2/hKLK3 mice but not in wild-type mice. 

Protein expression analysis

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Strain specific KLK3 expression analysis in homozygous B-hKLK2/hKLK3 mice by ELISA. Serum were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hKLK2/hKLK3 mice (H/H; H/H) and analyzed by ELISA with species-specific KLK3 ELISA kit. Human KLK3 was exclusively detectable in homozygous B-hKLK2/hKLK3 mice but not in wild-type mice.