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B-hRNASE4 mice
Strain Nam
C57BL/6-Rnase4tm1(RNASE4)Bcgen/Bcgen
Common Name  B-hRNASE4 mice
Background C57BL/6 Catalog number 112595
Aliases 

RNASE4:RAB1, RNS4

mRNA expression analysis

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Strain specific analysis of RNASE4 mRNA expression in wild-type C57BL/6 mice and B-hRNASE4 mice by RT-PCR. Lung RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hRNASE4 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human RNASE4 primers. Mouse Rnase4 mRNA was detectable only in wild-type C57BL/6 mice. Human RNASE4 mRNA was detectable only in homozygous B-hRNASE4 mice but not in wild-type mice.

Protein expression analysis



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Western blot analysis of RNASE4 protein expression in homozygous B-hRNASE4 mice. Various tissue lysates were collected from C57BL/6N wildtype (+/+) mice and homozygous B-hRNASE4 (H/H) mice, and then analyzed by western blot with anti-RNASE4 antibody. 40 μg total proteins were loaded for western blotting analysis. RNASE4 was detected in bladder.