top
Please input keywords
Order
*Country
한국
미국
일본
중국
영국
호주
프랑스
독일
이탈리아
싱가포르
인도
러시아
캐나다
스위스
기타 국가
*Province
*City
*Name
*Telephone
*Company
*Position
*Email
*Verification code
*Verification Code
B-hSORT1 mice plus
Strain Name C57BL/6N-Sort1tm2(SORT1)Bcgen/Bcgen Common Name  B-hSORT1 mice plus
Background C57BL/6N Catalog number 112493
Aliases 

Gp95, LDLCQ6, NT3, NTR3

mRNA expression analysis

from clipboard

Strain specific analysis of SORT1 mRNA expression in wild-type C57BL/6 mice and B-hSORT1 mice plus by RT-PCR. Brain RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hSORT1 mice plus (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human SORT1 primers. Mouse Sort1 mRNA was detectable in wild-type C57BL/6 mice. Human SORT1 mRNA was detectable only in homozygous B-hSORT1 mice plus but not in wild-type mice. 

Protein expression analysis

from clipboard

Strain specific SORT1 expression analysis in wild-type C57BL/6 mice and homozygous B-hSORT1 mice plus by western blot. Lung, brain, kidney and testicle tissue were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSORT1 mice plus (H/H) and analyzed by western blot with anti-SORT1 antibody. Mouse SORT1 was detectable only in wild-type mice. Human SORT1 was detectable only in homozygous B-hSORT1 mice plus.