Strain specific analysis of SORT1 mRNA expression in wild-type C57BL/6 mice and B-hSORT1 mice plus by RT-PCR. Brain RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hSORT1 mice plus (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human SORT1 primers. Mouse Sort1 mRNA was detectable in wild-type C57BL/6 mice. Human SORT1 mRNA was detectable only in homozygous B-hSORT1 mice plus but not in wild-type mice. 

Strain specific SORT1 expression analysis in wild-type C57BL/6 mice and homozygous B-hSORT1 mice plus by western blot. Lung, brain, kidney and testicle tissue were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSORT1 mice plus (H/H) and analyzed by western blot with anti-SORT1 antibody. Mouse SORT1 was detectable only in wild-type mice. Human SORT1 was detectable only in homozygous B-hSORT1 mice plus.