B-hTNFA mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

Strain Name	C57BL/6-Tnf^{tm1(TNF)Bcgen}/Bcgen	Common Name	B-hTNFA mice
Background	C57BL/6	Catalog number	110002
Related Genes	TNF (tumor necrosis factor, TNF-α)
NCBI Gene ID	21926

Targeting strategy

Gene targeting strategy for B-hTNFA mice. The targeted mouse Tnf whole genomic sequence including 5'UTR, 3'UTR and coding region was replaced by human TNF whole genomic sequence in B-hTNFA mice.

Protein expression analysis

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Strain specific TNFα expression analysis in homozygous B-hTNFA mice by ELISA

Serum were collected from WT and homozygous B-hTNFA (H/H) mice stimulated with LPS in vivo, and analyzed by ELISA with species-specific TNFα ELISA kit. Mouse TNFα was detectable in WT mice. Human TNFα was exclusively detectable in homozygous B-hTNFA but not WT mice.