top
Please input keywords
Order
*Country
한국
미국
일본
중국
영국
호주
프랑스
독일
이탈리아
싱가포르
인도
러시아
캐나다
스위스
기타 국가
*Province
*City
*Name
*Telephone
*Company
*Position
*Email
*Verification code
*Verification Code
B-hMICA mice
Strain Name
C57BL/6-Igs2tm1(MICA)Bcgen/Bcgen 
Common Name  B-hMICA mice
Background C57BL/6 Catalog Number 112161
Aliases 
MIC-A, PERB11.1
NCBI Gene ID
100507436
Description

  • This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. 
  • The human gene coding sequence was inserted to mouse Hipp11(H11) allele in B-hMICA mice. 
  • Human MICA was exclusively detectable on T cells, B cells and NK cells of homozygous B-hMICA mice.
  • Application: For example, This product is used for pharmacodynamics and safety evaluation of cancer.

Mechanism of action

from clipboard

Dual role of MICA/B as target molecule for immunosurveillance by NK cells and as mediator of tumor immune escape. 

  • Immunosurveillance: MICA/B expressed on the cell surface of tumor cells can be recognized by NK cells through NKG2D and promote a cytotoxic response that leads to tumor cell elimination.
  • Immunoevasion: MICA/B can associate with the ERp5 chaperone and, through a proteolytic cleavage mediated by ADAM10, ADAM17 and MMP14, generate sMICA/B that promote NKG2D down-regulation and impairment of NK cell-effector functions, thus facilitating tumor immune escape.


Protein expression analysis in spleen T, B and NK cells


from clipboard


Strain specific MICA expression analysis in wild type C57BL/6 mice and homozygous humanized B-hMICA mice by flow cytometry. Splenocytes were collected from wild type C57BL/6 mice (+/+) and homozygous B-hMICA mice (H/H), stimulated with PMA+ionomycin cocktail  in vitro ( Cells were cultured in presence of PMA/I for 2 h, extensively washed, and subsequently cultured for up to 22 h) and analyzed by flow cytometry with species-specific MICA/MICB antibody. Human MICA was exclusively detectable on T cells, B cells and NK cells of homozygous B-hMICA mice.