B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

Please input keywords

Order

*Country

한국

미국

일본

중국

영국

호주

프랑스

독일

이탈리아

싱가포르

인도

러시아

캐나다

스위스

기타 국가

*Province

*City

*Name

*Telephone

*Company

*Position

*Email

*Verification code

*Verification Code

Home

동물/세포 모델

동물/세포모델

Humanized Mouse Models

Humanizad Mouse Models

Text

B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice

Strain Name	C57BL/6N-Tg(CH17-302I14)1Bcgen, Siglece^tm1Bcgen/Bcgen	Common Name	B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice
Background	C57BL/6N	Catalog Number	112767
Aliases	CD328, AIRM-1, CDw328, D-siglec, SIGLEC-7; CD329, CDw329, FOAP-9, siglec-9; NA
NCBI Gene ID	27036, 5156, NA

Description

Sialic acid-binding Ig-like lectin 7 is a protein that in humans is encoded by the SIGLEC7 gene. Sialic acid-binding Ig-like lectin 9 is a protein that in humans is encoded by the SIGLEC9 gene.
The BAC containing whole human SIGLEC7 and SIGLEC9 genome sequence was inserted into mouse genome randomly in B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice. The mouse Siglec-E was knocked out B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice.
Human SIGLEC7 and SIGLEC9 were exclusively detectable in NK, T, B, DC, granulocytes, monocytes and macrophages cells of B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice. Mouse Siglec-E was not detectable in NK, B, T, DCs, granulocytes, monocytes or macrophages cells of B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice.
B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice provide a powerful preclinical model for in vivo evaluation of anti–Siglec-7 and Siglec-9 antibodies.
Application: For example, this product is used for pharmacodynamics and safety evaluation of anti–Siglec-7 and Siglec-9 antibodies for cancers.

Protein expression analysis in spleen NK cells

from clipboard

Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice. Spleen was collected from wild-type mice (+/+) and B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody(Biolegend, 339203) and anti-hSiglec-9 antibody(Biolegend, 351503). Human SIGLEC7 and SIGLEC9 were exclusively detectable in NK cells of B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice.

Protein expression analysis in spleen T cells

from clipboard

Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice. Spleen was collected from wild-type mice (+/+) and B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody(Biolegend, 339203) and anti-hSiglec-9 antibody(Biolegend, 351503). Human SIGLEC7 and SIGLEC9 were exclusively detectable in T cells of B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice.

Protein expression analysis in spleen B cells

from clipboard

Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice. Spleen was collected from wild-type mice (+/+) and B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody(Biolegend, 339203) and anti-hSiglec-9 antibody(Biolegend, 351503). Human SIGLEC7 and SIGLEC9 were exclusively detectable in B cells of B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice.

Protein expression analysis in spleen DC cells

from clipboard

Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice. Spleen was collected from wild-type mice (+/+) and B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody(Biolegend, 339203) and anti-hSiglec-9 antibody(Biolegend, 351503). Human SIGLEC7 and SIGLEC9 were exclusively detectable in DC cells of B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice.

Protein expression analysis in spleen granulocytes cells

from clipboard

Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice. Spleen was collected from wild-type mice (+/+) and B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody(Biolegend, 339203) and anti-hSiglec-9 antibody(Biolegend, 351503). Human SIGLEC7 and SIGLEC9 were exclusively detectable in granulocytes cells of B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice.

Protein expression analysis in spleen monocytes cells

from clipboard

Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice. Spleen was collected from wild-type mice (+/+) and B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody(Biolegend, 339203) and anti-hSiglec-9 antibody(Biolegend, 351503). Human SIGLEC7 and SIGLEC9 were exclusively detectable in monocytes cells of B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice.

Protein expression analysis in spleen macrophages cells

from clipboard

Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice. Spleen was collected from wild-type mice (+/+) and B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody(Biolegend, 339203) and anti-hSiglec-9 antibody(Biolegend, 351503). Human SIGLEC7 and SIGLEC9 were exclusively detectable in macrophages cells of B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice.

Protein expression analysis in spleen B, T and NK cells

from clipboard

Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice. Spleen was collected from wild-type mice (+/+) and B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice, and analyzed by flow cytometry with species-specific species-specific anti-mSiglec-E(Biolegend, 677106). Mouse Siglec-E was not detectable in B, T and NK cells of B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice or wild-type mice.

Protein expression analysis in spleen DCs and granulocytes cells

from clipboard

Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice. Spleen was collected from wild-type mice (+/+) and B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice, and analyzed by flow cytometry with species-specific species-specific anti-mSiglec-E(Biolegend, 677106). Mouse Siglec-E was detectable in DCs and granulocytes of wild-type mice, but not in DCs or granulocytes of B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice.

Protein expression analysis in spleen monocytes and macrophages cells

from clipboard

Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice. Spleen was collected from wild-type mice (+/+) and B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice, and analyzed by flow cytometry with species-specific species-specific anti-mSiglec-E(Biolegend, 677106). Mouse Siglec-E was detectable in macrophages of wild-type mice, but not in monocytes or macrophages B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice.

Frequency of leukocyte subpopulations in spleen

from clipboard

Frequency of leukocyte subpopulations in spleen by flow cytometry. Splenocytes were isolated from wild-type C57BL/6 mice and homozygous B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice (female, n=3, 6-week-old). A. Flow cytometry analysis of the splenocytes was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, B cells, NK cells, DCs, granulocytes, monocytes, macrophages, CD4+ T cells, CD8+ T cells and Tregs in B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice were similar to those in C57BL/6 mice, demonstrating that transgene of human SIGLEC7 and SIGLEC9 does not change the frequency or distribution of these cell types in spleen. The frequency of leukocyte subpopulations in blood and lymph nodes of B-Tg(hSIGLEC7/hSIGLEC9), Siglec-E KO mice were also comparable to wild-type C57BL/6 mice (Data not shown). Values are expressed as mean ± SEM. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***P < 0.001.