top
Please input keywords
Order
*Country
한국
미국
일본
중국
영국
호주
프랑스
독일
이탈리아
싱가포르
인도
러시아
캐나다
스위스
기타 국가
*Province
*City
*Name
*Telephone
*Company
*Position
*Email
*Verification code
*Verification Code
B-hBTLA/hHVEM mice
Strain Name
C57BL/6-Btlatm1(BTLA)Bcgen Tnfrsf14tm1(TNFRSF14)Bcgen/Bcgen   
Common Name  B-hBTLA/hHVEM mice
Background C57BL/6 Catalog number 121080
Aliases 

BTLA, BTLA1, CD272, B and T lymphocyte associated; TNFRSF14, HVEM, ATAR, CD270, HVEA,

HVEM, LIGHTR, TR2, Herpesvirus entry mediator, TNF receptor superfamily member 14


mRNA expression analysis




Strain specific analysis of BTLA and HVEM gene expression in wild type (WT) mice and B-hBTLA/hHVEM mice by RT-PCR.

Mouse Btla and Hvem mRNA was detectable only in splenocytes of WT mice (+/+). Human BTLA and HVEM mRNA was detectable only in homozygous B-hBTLA/hHVEM mice (H/H;H/H) but not in WT mice (+/+).


Protein expression analysis in B cells





Strain specific BTLA  expression analysis in homozygous B-hBTLA/hHVEM mice by flow cytometry.

Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hBTLA/hHVEM mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-BTLA antibody. Mouse BTLA  was detectable in WT mice (+/+). Human BTLA was exclusively detectable in homozygous B-hBTLA/hHVEM mice (H/H;H/H) but not in WT mice (+/+).


Protein expression analysis in T cells




Strain specific HVEM  expression analysis in homozygous B-hBTLA/hHVEM mice by flow cytometry.

Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hBTLA/hHVEM mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-HVEM antibody. Mouse HVEM  was detectable in WT mice (+/+). Human HVEM was exclusively detectable in homozygous B-hBTLA/hHVEM mice (H/H;H/H) but not in WT mice (+/+).


Tumor growth curve & Body weight changes


from clipboard


Subcutaneous homograft tumor growth of B-hHVEM MC38 cells. B-hHVEM MC38 cells (1x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into homozygous B-hBTLA/hHVEM mice (female, 6-week-old, n=7). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean ± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hHVEM MC38 cells were able to form tumors in vivo and can be used for efficacy studies.

Tumor growth curve of individual mice

from clipboard

B-hHVEM MC38 tumor cells growth of individual mice. B-hHVEM MC38 cells (1x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into homozygous B-hBTLA/hHVEM mice (female, 6-week-old, n=7). As shown in panel, B-hHVEM MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.