B-hCD22 mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

Strain Name	C57BL/6-Cd22^{tm1(CD22)Bcgen}/Bcgen	Common Name	B-hCD22 mice
Background	C57BL/6	Catalog number	110775
Related Genes	cluster of differentiation-22, SIGLEC2
NCBI Gene ID	12483

mRNA expression analysis

Strain specific analysis of CD22 gene expression in WT and homozygous B-hCD22 mice by RT-PCR. Mouse Cd22 mRNA was detectable in splenocytes of wild-type (+/+) mice. Human CD22 mRNA was detectable only in H/H, but not in +/+ mice.

protein expression analysis

Strain specific CD22 expression analysis in homozygouse B-hCD22 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCD22 (H/H) mice, and analyzed by flow cytometry with species-specific CD22 antibody. Mouse CD22 was detectable in WT mice. Human CD22 was exclusively detectable in homozygous B-hCD22 but not WT mice.